AffiPCR® 2X EpiArt HS Taq Master Mix
AffiPCR® 2X EpiArt HS Taq Master Mix is suitable for amplifying bisulfite-converted DNA, and contains EpiArt HS Taq DNA Polymerase, dNTP and an optimized buffer system. Compared with common DNA, bisulfite conversion results in severe DNA damage and affects PCR performance. This product uses the enzyme-engineered Hot-Start EpiArt HS Taq DNA Polymerase and an optimized buffer system to achieve superior amplification efficiency, sensitivity and specificity for bisulfite-converted DNA compared with common PCR reagents. The pre-formulated 2X Master Mix can be used for PCR reactions by simply adding primers and templates to reduce pipetting and improve throughput and the reproducibility of results. AFG-VZ-EM202 contains a blue dye, which can directly perform electrophores after PCR, thereby simplifying the operation. The PCR product has an adenine at the 3' end that can be cloned into the T vector. It is also compatible with ClonExpress and TOPO cloning kits.
Compenents:
| Compenent | AFG-VZ-EM201-01 | AFG-VZ-EM201-02 | AFG-VZ-EM201-03 |
| AffiPCR® 2X EpiArtTM HS Taq Master Mix | 1 mL | 5 x 1 mL | 15 x 1 mL |
Storage Conditions:
Store at -30 ~ -15°C and transport at ≤0°C.
Applications:
It is applicable for PCR with various templates such as genomic DNA, cDNA, plasmid DNA, dU-containing DNA, etc.
Notes:
For research use only. Not for use in diagnostic procedures.