AffiNGS® VAHTS Universal Plus DNA Library Prep Kit for MGI V2
AffiNGS® VAHTS Universal Plus DNA Library Prep Kit for MGI V2 is a fragment enzyme DNA library preparation kit developed specifically for the MGI sequencing platform. This was optimized and upgraded for the original version. which significantly reduced the proportion of Artificial Invert Chimera Reads in the FFPE sample DNA library and improved the reliability of SNV detection while maintaining the original high performance. This kit combines DNA fragmentation, end repair and dA tailing into one step. The product does not require purification. The adapter ligation, library enrichment and sorting can be performed directly. The 100 pg - 1 µg Input DNA can be converted into a dedicated library for MGI sequencing platforms. This kit is fully compatible with DNA from different sources and different amount of input DNA. The required library can be achieved simply by adjusting the fragmentation time according to the size of the target insert fragment. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.
Component:
| Components |
AFG-VZ-NDM627-01 (24 rxns) |
AFG-VZ-NDM627-02 (96 rxns) |
| FEA Buffer V2 | 120 µl | 480 µl |
| FEA Enzyme Mix V2 | 240 µl | 960 µl |
| Rapid Ligation Buffer V2 | 600 µl | 4 X 600 µl |
| Rapid DNA Ligase V2 | 120 µl | 480 µl |
| VAHTS HiFi Amplificaation Mix | 600 µl | 4 X 600 µl |
| PCR Primer Mix for MGI | 120 µl | 480 µl |
| Neutralization Buffer | 120 µl | 480 µl |
| Control DNA ( 100 ng/µl ) | 10 µl | 10 µl |
Control DNA is salmon genome DNA.
Storage:
Store at -30 ~ -15°C and transport at ≤0°C.