AffiAB® Goat anti-tdTomato Polyclonal IgG Antibody
AffiAB® Goat anti-tdTomato Polyclonal IgG Antibody provides fast and reliable titers of antibodies with high specificity, making it an ideal choice for study of tdTomato protein. The antibody has been rigorously tested to ensure accuracy and reliability in a range of laboratory settings.
Host: Goat
Species: N/A
Description:
The AffiAB® Goat Anti-tdTomato Polyclonal IgG Antibody is an antibody derived from goats that specifically recognizes and binds to the tdTomato protein. tdTomato is a red fluorescent protein commonly used as a marker in molecular and cellular biology research.
This polyclonal antibody is generated by immunizing goats with purified tdTomato protein, resulting in the production of antibodies that specifically target and bind to tdTomato. The antibody can be used in various applications, including immunohistochemistry, immunofluorescence, and Western blotting, to detect and visualize the expression and localization of tdTomato in cells and tissues.
tdTomato is widely used as a reporter gene and fusion tag in molecular biology studies. It exhibits bright red fluorescence, making it useful for visualizing gene expression, protein localization, and cell tracking. The AffiAB® Goat Anti-tdTomato Polyclonal IgG Antibody provides a reliable tool for researchers to detect and study tdTomato-tagged proteins in a variety of experimental settings.
When using the AffiAB® Goat Anti-tdTomato Polyclonal IgG Antibody, it is important to follow the recommended protocols and dilutions provided by the manufacturer. This ensures optimal performance and specific detection of tdTomato in your experiments. It is also essential to include appropriate controls and perform any necessary optimization steps to ensure reliable and accurate results.
For detailed instructions on the specific applications and usage of the AffiAB® Goat Anti-tdTomato Polyclonal IgG Antibody, please refer to the product datasheet or consult the manufacturer's instructions.
Gene Identifier/Accession Number: N/A
Form: N/A
Concentration: 2.5 mg/mL
Type: Primary
Clonality: Polyclonal
Isotype: IgG
Conjugation: Unconjugated
Application: Western Blot, Immunofluorescence, Immunohistochemistry PARAFFIN PROTOCOL , Immunohistochemistry FROZEN, Immunoelectron Microscopy.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.
Goat anti-tdTomato Polyclonal IgG Antibody
The application of fluorescent proteins like tdTomato in biological research has revolutionized our ability to visualize and study molecular processes in live cells. Central to these advancements is the use of specific antibodies, such as the Goat Anti-tdTomato Polyclonal IgG Antibody. This article explores the properties, applications, and impact of this antibody in fluorescent imaging and molecular biology.
Background:
tdTomato is a red fluorescent protein widely used as a reporter in cellular and molecular biology. It is favored for its brightness and photostability, making it an excellent tool for tracking gene expression, protein localization, and cell lineage studies. The development of specific antibodies against tdTomato, like the Goat Anti-tdTomato Polyclonal IgG, has further expanded its utility in research.
Properties of Goat Anti-tdTomato Polyclonal IgG Antibody:
Source and Production: This antibody is produced in goats by immunizing them with the tdTomato protein. The use of goats allows for the generation of high-affinity antibodies in large quantities.
Specificity and Sensitivity: The polyclonal nature of this antibody means it recognizes multiple epitopes on the tdTomato protein, enhancing its ability to detect the protein across different experimental conditions and applications.
Purity and Validation: The antibody is purified using affinity chromatography techniques to ensure high specificity. It undergoes extensive validation, including Western blotting, immunofluorescence, and ELISA, to confirm its reactivity and specificity to tdTomato.
Applications in Research:
Fluorescent Microscopy: The primary application of this antibody is in fluorescent microscopy, where it is used to enhance the visualization of tdTomato-tagged proteins or cells. It allows for more precise and detailed imaging, particularly in tissues or contexts where fluorescent protein expression is low.
Co-localization Studies: In studies where multiple proteins are tagged with different fluorescent markers, the Goat Anti-tdTomato antibody can be used to clearly distinguish tdTomato-tagged proteins from others, facilitating co-localization analysis.
Flow Cytometry and Sorting: The antibody is also useful in flow cytometry, enabling researchers to identify and sort cells expressing tdTomato with higher accuracy and efficiency.
Challenges and Considerations:
While the Goat Anti-tdTomato Polyclonal IgG Antibody is a powerful tool, it is important to consider potential issues like batch-to-batch variability and non-specific binding. Researchers must implement appropriate controls and confirm the specificity in their particular experimental setup. Ethical considerations in the production of antibodies from goats should also be observed.
Conclusion:
The Goat Anti-tdTomato Polyclonal IgG Antibody is a significant advancement in the field of fluorescent imaging and molecular biology. Its ability to specifically detect tdTomato-tagged proteins enhances the clarity and precision of fluorescence-based studies. As the use of fluorescent proteins continues to grow in biological research, the role of such specialized antibodies becomes increasingly vital, paving the way for more sophisticated and insightful scientific discoveries.