SKU: AFG-VZ-EN303-01

AffiZYME® T7 Endonuclease I

Vendor AffiGEN
Regular price 372,00 zł PLN
Sale price 372,00 zł PLN Regular price
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AffiZYME® T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5' to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.

Components

Component 250 U 1250 U
T7 Endonuclease I 25 μL 125 μL
T7 Endonuclease I Reaction Buffer (10 ×) 1 mL  mL
Control Template* 10 μL 20 μL

 

Storage Conditions:

Store at -30 ~ -15℃ and transport at ≤0℃.

Applications:

It's applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection.

Notes:

For research use only. Not for use in diagnostic procedures.

T7 endonuclease I is a structure-selective enzyme which acts on different DNA substrates with different activities.Therefore, it is essential to control the amount of enzyme and reaction time when cleaving a specific substrate. Nonspecific nuclease activity will be increased when the temperature is above 42℃. Control the temperature and make sure it won't exceed 55℃, or the activity of enzyme will be decreased.