AffiZYME® T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5' to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.
Components
| Component | 250 U | 1250 U |
| T7 Endonuclease I | 25 μL | 125 μL |
| T7 Endonuclease I Reaction Buffer (10 ×) | 1 mL | mL |
| Control Template* | 10 μL | 20 μL |
Storage Conditions:
Store at -30 ~ -15℃ and transport at ≤0℃.
Applications:
It's applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection.
Notes:
For research use only. Not for use in diagnostic procedures.
T7 endonuclease I is a structure-selective enzyme which acts on different DNA substrates with different activities.Therefore, it is essential to control the amount of enzyme and reaction time when cleaving a specific substrate. Nonspecific nuclease activity will be increased when the temperature is above 42℃. Control the temperature and make sure it won't exceed 55℃, or the activity of enzyme will be decreased.