AffiPCR® 1st Strand cDNA Synthesis Kit (+gDNA wiper) V2
AffiPCR® 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a dedicated kit for 1st strand cDNA synthesis that includes the removal step of genomic DNA. Compared with the previous generation of HiScript Reverse Transcriptase, the HiScript II Reverse Transcriptase in this kit has greatly improved thermal stability, template binding affinity and inhibitor tolerance. The gDNA wiper used in this kit can quickly remove genomic DNA contamination within 2 min, ensuring more reliable subsequent results and simplifying the design of qPCR primers without the need to span an exon-exon junction. 10X RT Mix contains optimized buffer system and dNTP; HiScript II Enzyme Mix contains HiScript II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in this product has the stronger anchoring ability of Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Oligo (dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. With flexible and optional subsequent steps, the kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning or efficiently synthesize the highly uniform cDNA for qPCR.
Components:
| Components | 50 Reactions | 100 Reactions |
| RNase-free ddH2O | 1 mL | 1 mL |
| 4 × gDNA wiper Mix | 200 μL | 400 μL |
| 10 × RT Mixa | 120 μL | 240 μL |
| HiScript II Enzyme Mixb | 100 μL | 200 μL |
| Oligo (dT)23VN (50 μM) | 50 μL | 100 μL |
| Random hexamers (50 ng/μl) | 50 μL | 100 μL |
Storage Conditions:
Store at -30°C ~ -15°C and transport at ≤0°C. 10 × RT Mix contains high concentration of DTT, which may precipitate at low temperature. Please restore to room temperature, shake gently and mix thoroughly, wait to redissolve the precipitation before use.
Applications:
It is applicable for reverse transcription reaction of animal, plant and microbial RNA.
Notes:
For research use only. Not for use in diagnostic procedures.
Prevent RNase contamination Please keep the experiment area clean; wear disposable gloves and masks; use RNase-free consumables such as centrifuge tubes and pipette tips.