AffiGEN® TransfectTM 1000 - Mammalian Cell
Product Description
AffiGEN® TransfectTM 1000 - Mammalian Cell is an advanced and enhanced version of the conventional calcium phosphate transfection reagent. Leveraging innovative chemistry, AffiGEN® TransfectTM 1000 features significantly reduced calcium concentrations, resulting in markedly decreased cytotoxicity while preserving the effectiveness of transfection. Moreover, it offers a reliable approach for generating both long-term stable and transient transfectants in a wide range of adherent cell lines. When compared to liposome and polymer-based transfection reagents, AffiGEN® TransfectTM 1000 stands out with exceptional efficiency, particularly on cell lines such as HepG2, HEK293, CHO, Hela, COS, 3T3, LNcap, C6, PC12, and primary cultured cells, among others. One unit of AffiGEN® TransfectTM 1000, with a volume of 1.0 ml, is suitable for more than 600 transfections in 24-well plates or more than 300 transfections in 6-well plates.
Application
AffiGEN® TransfectTM 1000 - Mammalian Cell is designed for high-efficiency stable or transient transfection of eukaryotic cells.
Features
- Exceptionally efficient: Components meticulously optimized for achieving high transfection efficiencies.
- Streamlined procedures: The entire process is consolidated into a single-tube reaction.
Storage Condition
Store AffiGEN® TransfectTM 1000 - Mammalian Cell at 4°C. When stored under proper conditions, the product maintains stability for 12 months or longer.
DNA In Vitro Transfection Reagents Specifications
| Product | Nucleic Acid | Type | Delivery Method | Toxicity | Efficiency | Serum Tolerance |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | DNA | Calcium phosphate | Precipitation | High | High | High |
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | DNA, siRNA | Polymer | Polymer complex | Low | High | Low |
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | DNA, siRNA | Polymer | Polymer complex | Moderate | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | DNA | Polymer | Polymer complex | Low | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 3000 | DNA | Liposomal | Liposome-mediated | Moderate | High | High |
| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | DNA, siRNA | Liposomal | Liposome-mediated | Very low | High | High |
| AffiGEN® Transfect™ DNA In Vitro 5000 | DNA, siRNA | Polymer | Polymer complex | Moderate | High | High |
DNA In Vitro Transfection Reagents Application Guide
| Product | Applications | Cell Types | Cell lines | Suitable for | Additional Notes |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | Protein production, stable cell line generation, viral vector production | Adherent, some suspension | HEK293, HepG2, CHO | Large-scale transfection, cost-effective |
Lower calcium concentration than traditional methods
|
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Sensitive cell lines, maximizing cell viability |
Multiple formulations available
|
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Balancing efficiency and viability |
Released from DNA complex
|
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | Protein production, viral vector production | Most, including sensitive | Various | Long DNA, high efficiency |
Proprietary polymer
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| AffiGEN® Transfect™ DNA In Vitro 3000 | Viral vector production, antibody production, protein production | Adherent, suspension | Various | Lentivirus production, high efficiency |
Enhanced conjugate
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| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | Protein production, gene editing, virus production | Most, including sensitive | Various | Low toxicity, high efficiency |
Fluorinated cationic lipids
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| AffiGEN® Transfect™ DNA In Vitro 5000 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Common cell lines, biodegradable |
Biodegradable polycationic polymer
|