AffiAB® Anti-dsRNA Monoclonal Antibody K2 (IgM)
Description
AffiAB® Anti-dsRNA Monoclonal Antibody K2 is a mouse-derived IgM antibody that specifically recognizes double-stranded RNA (dsRNA) structures of ≥40 base pairs. Sequence and nucleotide composition of the dsRNA do not affect binding, and the antibody reliably detects a wide range of natural and synthetic dsRNAs, including poly(I)-poly(C) and poly(A)-poly(U). K2 is highly recommended for use in (sandwich) ELISA formats and serves as an isotype alternative to J2 and K1 antibodies, particularly useful in multiplex detection strategies.
Formulation
- Form: Undiluted hybridoma cell culture supernatant
- Concentration: Not specified (use as supplied)
- Volume: ≤2 mL (centrifuge briefly before opening)
Applications
- Sandwich ELISA (recommended dilution 1:2 in PBS)
- Immunohistochemistry (IHC)
- Dot blot
Specificity
- Recognizes dsRNA helices ≥40 bp
- Sequence- and nucleotide composition-independent
- Validated against ~40–50 naturally occurring dsRNAs
- Binds synthetic dsRNAs (poly(I):poly(C), poly(A):poly(U))
- High avidity binding as demonstrated in Schönborn et al., 1991
Species and Isotype
- Species origin: Mouse
- Heavy chain: IgM
- Light chain: Kappa
Storage and Handling
- Shipping: Shipped on dry ice
- Storage:
- Upon receipt: store at -20 °C to -80 °C
- Upon reconstitution: aliquot and avoid freeze/thaw cycles
- Shelf life: 12 months from delivery date
Quality Control
Functionality validated via antigen ELISA (AN-ELISA), benchmarked against reference K2