AffiPCR® Full Script cDNA Kit with gDNA Removal
AffiPCR® Full Script cDNA Kit with gDNA Removal is high-efficiency reverse transcription kit optimized for full-length cDNA synthesis, suitable for a broad range of applications including RT-qPCR and cloning. Includes built-in genomic DNA removal and flexible priming options.
Key Features:
- Synthesizes full-length cDNA up to 20 kb
- Includes Oligo(dT)₂₀VN and Random Hexamers
- Effective gDNA removal in 2 minutes
- Compatible with low-quality or degraded RNA
- Ideal for cloning, gene expression profiling, and general RT workflows
Components:
| Component |
AFG-M-0173 50 Reactions |
AFG-M-0174 100 Reactions |
| RNase-free ddH2O | 1 ml | 1 ml |
| 5X gDNA Removal Mix | 100 µl | 200 µl |
| 10X RT Mixa | 100 µl | 200 µl |
| Full Script Enzyme Mixb | 100 µl | 200 µl |
| Oligo (dT)20 VN | 50 µl | 100 µl |
| Random hexamers | 50 µl | 100 µl |
a. It contains dNTPs.
b. It contains RNase inhibitor.
Storage:
Store at -30 ~ -15°C and transport at ≤0°C. 10X RT Mix contains high concentration of DTT, which may precipitate at low temperature. Please restore to room temperature, shake gently and mix thoroughly, wait to redissolve the precipitation before use.
Equivalence:
| AFG-M-0173/4 | AffiGEN AffiPCR® Full Script cDNA Kit with gDNA Removal |
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| FSK-101 | TOYOBO ReverTra Ace -ɑ-® |
| E3010 | NEB LunaScript™ RT SuperMix Kit |
| RR047 | TaKaRa PrimeScrip™ RT reagent Kit with gDNA Eraser (Perfect Real Time) |
| 1708841 | Bio-Rad iScript™ Reverse Transcription Supermix for RT-qPCR |