AffiCHECK® Human Herpesvirus 8 (HHV-8) DNA PCR Quantitative Positive Control
Product Datasheet - AFG-CHK-0507
PRODUCT DESCRIPTION:
AffiCHECK® Human Herpesvirus 8 (HHV-8) DNA PCR Quantitative Positive Control is a high-quality and reliable reference standard containing known concentrations of HHV-8 DNA. It is formulated using modified, non-infectious viral material to ensure both safety and stability. This control is specifically designed to support accurate and consistent results in quantitative PCR (qPCR) applications.
INTENDED USE:
AffiCHECK® Human Herpesvirus 8 (HHV-8) DNA PCR Quantitative Positive Control serves as a comprehensive process control for evaluating the performance of nucleic acid amplification tests that detect and quantify Human Herpesvirus 8 (HHV-8) DNA. It is essential for validating the accuracy and reliability of PCR assays in clinical, research, and diagnostic settings.
PRODUCT FEATURES:
- Specificity: Designed specifically for the detection of Human Herpesvirus 8 (HHV-8) DNA.
- Formulation: Lyophilized to ensure extended shelf life and stability.
- Convenience: Ready-to-use immediately after reconstitution.
- Compatibility: Suitable for a wide range of PCR assays.
CHARACTERISTICS:
- Cultivation & Extraction: Cultivated in BC-3 cells then extracted using a reliable genomic DNA method.
- Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
- Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.
PERFORMANCE CHARACTERISTICS:
- Sensitivity: Optimized for high sensitivity in PCR detection of HHV-8 DNA.
- Specificity: Exclusively detects Human Herpesvirus 8 (HHV-8) DNA minimizing cross-reactivity.
- Reproducibility: Consistent results across different test runs and batches.
PACK CONTENT :
| Components | Pack Size | Note |
| DNA Control Lyophilized Powder (1x104 to 2x104 Copies/µL) | 1 Vial | Quantified by Real-Time PCR. |
| Buffer | 500 µL | Water, DNase, RNase Free. |
PRECAUTIONS:
- For research use or diagnostic control purposes only.
- Follow standard laboratory practices and use PPE.
- Use Sterile Filter Tips to prevent contamination.
- Handle infectious specimens with precautions.
- Disinfect work surfaces.
- Maintain separate working areas for testing.
- Dispose of unused reagents and waste as per regulations.
- May contain genetic material, handle with care.
- No method ensures total absence of infectious agents.
- Follow local waste disposal rules.
- Dilute below 103 copies/µL immediately before use.
- Avoid freezing for low-copy dilutions to prevent DNA degradation.
INSTRUCTIONS FOR USE:
- Open the foil pouch of AffiCHECK® Human Herpesvirus 8 (HHV-8) DNA.
- Centrifuge AffiCHECK® Human Herpesvirus 8 (HHV-8) DNA Lyophilized Powder at 1000 g for 1 minute.
- Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
- The concentration will be 1x104 to 2x104 Copies/µL once reconstituted.
- Vortex for 1 min for complete dissolution and homogenization.
RECOMMENDED STORAGE:
- Before Reconstitution: Store at 2-8ºC.
- After Reconstitution: Store at -20ºC.
- Shipping: No special instructions
- Shelf Life: 12 months from production date.
ORDERING INFORMATION :
| Catalog Number | Description | Size |
| AFG-CHK-0507 | AffiCHECK® Human Herpesvirus 8 (HHV-8) DNA PCR Quantitative Positive Control | 1 vial + 500 µL Buffer |