AffiAB® Goat Anti-CD45 Polyclonal IgG Antibody
Our Goat Anti-CD45 Polyclonal IgG Antibody specifically targets the CD45 antigen for recognition and binding. Ideal for ELISA, Western Blot, and Immunohistochemical assays, this polyclonal antibody ensures reliable performance and accuracy.
Host: Goat
Species: Human
Description:
Goat anti-CD45 polyclonal IgG antibody is an antibody that specifically binds to CD45, also known as leukocyte common antigen (LCA). CD45 is a transmembrane protein that is widely expressed in all hematopoietic cells and is involved in signal transduction in immune cells. It plays a crucial role in the activation and proliferation of T and B lymphocytes, and its expression is used to distinguish between lymphoid and myeloid cells. Goat anti-CD45 polyclonal IgG antibody is commonly used as a tool in research to detect and analyze CD45 expression in cells.
Gene Identifier/Accession Number: ENSG00000081237
Form: N/A
Concentration: 2.5 mg/mL
Type: Primary
Clonality: Polyclonal
Isotype: IgG
Conjugation: Unconjugated
Application: Western Blot
Storage: For continuous use, store at 2-8 °C for one-two days. For extended storage, store in -20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.
Goat Anti-CD45 Polyclonal IgG Antibody
The Goat Anti-CD45 Polyclonal IgG Antibody has emerged as a pivotal tool in immunological research. CD45, a transmembrane protein tyrosine phosphatase, is widely recognized as a critical marker for leukocytes (white blood cells) and plays a significant role in cell-mediated immune responses. This antibody's ability to specifically target CD45 has profound implications in research areas spanning from immunology to hematological diseases.
Understanding Goat Anti-CD45 Polyclonal IgG Antibody
Production and Specificity:
This antibody is generated by immunizing goats with a portion of the CD45 antigen. The resulting polyclonal IgG antibodies have the capacity to recognize multiple epitopes on CD45, enhancing detection sensitivity and specificity. This polyclonality is advantageous for comprehensive detection in various experimental contexts.
Benefits of Goat-Derived Antibodies:
Goats, as a source for antibody production, offer several benefits including high antigen sensitivity and substantial antibody yields. Their broad immune response is particularly effective for producing antibodies against diverse antigens, like CD45, which may exhibit variations in different cell types or species.
Applications in Research
1. Immunophenotyping:
A primary application of the Goat Anti-CD45 Polyclonal IgG Antibody is in immunophenotyping, where it helps identify and classify different types of leukocytes based on their surface markers. This is crucial in both basic immunology research and clinical diagnostics.
2. Hematological Disease Studies:
In the study of hematological diseases such as leukemia and lymphoma, this antibody plays a key role in identifying aberrations in leukocyte populations, aiding in diagnosis and monitoring disease progression.
3. Immunohistochemistry (IHC):
The antibody is widely used in IHC to detect CD45 expression in tissue samples, providing insights into the immune cell distribution and status within various tissues, which is essential in understanding inflammatory and autoimmune diseases.
4. Flow Cytometry:
In flow cytometry, the Goat Anti-CD45 Polyclonal IgG Antibody allows for the effective labeling and analysis of leukocyte populations, facilitating studies in immune cell dynamics and function.
5. Western Blotting:
This antibody is also employed in Western blotting for the detection and analysis of CD45 expression in cell lysates, contributing to a deeper understanding of immune signaling pathways.
Conclusion
The Goat Anti-CD45 Polyclonal IgG Antibody is an indispensable tool in immunological research and diagnostics. Its specificity to CD45 and the broad reactivity provided by its polyclonal nature make it invaluable for a wide range of applications, from basic research to clinical diagnostics. As our understanding of the immune system continues to grow, this antibody will undoubtedly remain central to future breakthroughs in immunology and related fields.