AffiPCR® SYBR qPCR Master Mix (High ROX Premixed)

AffiPCR® SYBR qPCR Master Mix (High ROX Premixed)

AffiPCR® SYBR qPCR Master Mix (High ROX Premixed) is a special premix for qPCR assays based on SYBR Green I fluorescence method. The core component, Champagne Taq DNA Polymerase, is a novel antibody-modified hot-start DNA polymerase with high specificity and detection sensitivity. Equipped with the optimized buffer for qPCR and specificity-promoting factors, it is very suitable for highly specific and sensitive qPCR assays. This product is a 2X premix reagent that contains SYBR Green I at an optimal concentration for qPCR. It can obtain a good standard curve in a wide quantitative range, and accurately quantify target genes with good repeatability and high reliability.

Components:

Applications:

It is applicable for the amplification and quantification of DNA samples from all species, including genomic DNA, cDNA, plasmid DNA and λDNA.

Applicable Instruments:

This product is premixed with ROX Reference Dye 2 (high concentration) to correct the fluorescence signal error between wells. It is suitable for the following qPCR instruments:

Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOnePlus; and other qPCR instruments that need to add high concentration of ROX Reference Dye.

Storage Conditions:

Store at -30°C ~ -15°C and protect from light. Transport at ≤0°C. After thawing, the Master mix can be stored stably for 6 months at 2°C ~ 8°C and protected from light.

Equivalence: