AffiPCR® Bst II Pro DNA Polymerase Large Fragment

AffiPCR® Bst II Pro DNA Polymerase Large Fragment

AffiPCR® Bst II Pro DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with Bst II DNA Polymerase Large Fragment (AffiGEN #AFG-VZ-P702), Bst II Pro DNA Polymerase Large Fragment combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymerase activity at temperatures above 50°C. It displays superior amplification speed, specificity, salt tolerance and thermal stability. The reaction system can be prepared at room temperature.

Storage Conditions:

Store at -30°C ~ -15°C and transport at ≤0°C.

Applications:

It is applicable for isothermal amplifications such as LAMP, HDA and RCA.

Source:

Bst II Pro DNA Polymerase Large Fragment comes from Bacillus stearothermophilus.

Notes:

For research use only. Not for use in diagnostic procedures.