AffiDNA® Human Methylated DNA Control
This AffiDNA® Human Methylated DNA Control is a reliable tool for validating DNA methylation assays. With a known percentage of methylated and unmethylated DNA, it provides accurate results for assessing assay sensitivity and specificity. Save time and resources by using this quality control for your methylation studies.
Applications of AffiDNA® Human Methylated DNA Control
Methylation-Specific PCR (MSP):
AffiDNA® Human Methylated DNA Control is crucial for validating methylation-specific PCR assays. It provides a known methylated DNA template to test the specificity and sensitivity of primers designed to amplify methylated DNA sequences. This ensures the accurate detection of methylation status in experimental samples.
Bisulfite Conversion Efficiency Testing:
In bisulfite sequencing, this control DNA helps to verify the efficiency of the bisulfite conversion process. It serves as a standard to confirm that unmethylated cytosines are fully converted to uracil, while methylated cytosines remain unchanged, ensuring accurate methylation analysis.
Quantitative Methylation Analysis:
AffiDNA® Human Methylated DNA Control is ideal for use as a reference in quantitative methylation assays such as pyrosequencing, methylation-specific quantitative PCR (qMSP), and droplet digital PCR (ddPCR). It allows for the calibration and normalization of methylation levels, facilitating precise quantification of methylation in target regions.
Next-Generation Sequencing (NGS) for Epigenomics:
This control DNA is useful in next-generation sequencing workflows focused on epigenomics. It helps in validating the accuracy and consistency of methylation sequencing protocols, from library preparation to data analysis, ensuring high-quality and reliable results.
Epigenetic Biomarker Validation:
Researchers developing and validating epigenetic biomarkers can use AffiDNA® Human Methylated DNA Control as a standard to test the performance of their assays. It provides a consistent methylation profile for assessing the sensitivity and specificity of biomarker detection methods.
Differential Methylation Analysis:
This product can be used in studies aimed at identifying differentially methylated regions (DMRs) between different biological conditions or sample groups. It serves as a control to ensure that observed differences in methylation are accurate and not due to technical variability.
Assay Development for Clinical Diagnostics:
In the development of clinical diagnostic assays for detecting diseases associated with DNA methylation changes (e.g., cancer), this control DNA is essential. It provides a benchmark for validating the diagnostic performance of new assays, ensuring they can reliably detect clinically relevant methylation patterns.
Training and Proficiency Testing:
AffiDNA® Human Methylated DNA Control is valuable for training laboratory personnel and conducting proficiency testing. It helps in demonstrating and practicing techniques for methylation analysis, ensuring that staff are proficient in using these methods.
Methylation Array Validation:
For researchers using methylation arrays (e.g., Illumina Infinium MethylationEPIC BeadChip), this control DNA serves as a standard to verify the performance of the array platform. It ensures the accurate detection and quantification of methylation levels across a wide range of CpG sites.
Quality Control in Epigenetic Research:
In epigenetic research, maintaining high-quality standards is essential. AffiDNA® Human Methylated DNA Control acts as a quality control measure, ensuring that experimental procedures and reagents produce consistent and reproducible methylation data.
Comparative Studies on Methylation Techniques:
Researchers comparing different methylation detection techniques can use this control DNA to benchmark and evaluate the performance of various methods. It provides a consistent methylation profile for assessing the advantages and limitations of each technique.
By incorporating AffiDNA® Human Methylated DNA Control into these applications, researchers can ensure the accuracy, reliability, and reproducibility of their methylation studies, enhancing the overall quality of epigenetic research and diagnostic assays.