SKU: AFG-CHK-0557

AffiCHECK® Salmonella typhi DNA - PCR Qualitative Positive Control

Vendor AffiGEN
Regular price £474.00 GBP
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AffiCHECK® Salmonella typhi DNA - PCR Qualitative Positive Control

Product Datasheet - AFG-CHK-0557

PRODUCT DESCRIPTION:

AffiCHECK® Salmonella typhi DNA - PCR Qualitative Control is a high-quality, reliable standard containing known quantities of Salmonella typhi DNA. It's designed using modified and non-infectious bacteria to ensure stability, thereby contributing to accurate and reliable results in PCR applications.

INTENDED USE:

AffiCHECK® Salmonella typhi DNA - PCR Qualitative Control serves as a comprehensive process control for the evaluation of the performance of nucleic acid tests for determination of the presence of Salmonella typhi nucleic acid. It's essential in validating the performance of PCR assays in clinical, research, and diagnostic settings.

PRODUCT FEATURES:

  • Specificity: Tailored for Salmonella typhi DNA detection.
  • Formulation: Lyophilized for enhanced stability.
  • Convenience: Ready-to-use upon reconstitution.
  • Compatibility: Suitable for a wide range of PCR assays.

CHARACTERISTICS:

  • Cultivation & Extraction: Cultivated in MacConkey agar and extracted using a reliable genomic DNA method.
  • Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
  • Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.

PERFORMANCE CHARACTERISTICS:

  • Sensitivity: Optimized for high sensitivity in PCR detection.
  • Specificity: Exclusively detects Salmonella typhi DNA.
  • Reproducibility: Consistent results across different test runs and batches.

PACK CONTENT :

Components Pack Size Note 
DNA Control Lyophilized Powder (1x104 to 2x104 Copies/µL) 1 Vial Quantified by Real-Time PCR.
Buffer 500 µL Water, DNase, RNase Free.

PRECAUTIONS:

  • For research use or diagnostic control purposes only.
  • Follow standard laboratory practices and use PPE.
  • Use Sterile Filter Tips to prevent contamination.
  • Handle infectious specimens with precautions.
  • Disinfect work surfaces.
  • Maintain separate working areas for testing.
  • Dispose of unused reagents and waste as per regulations.
  • May contain genetic material, handle with care.
  • No method ensures total absence of infectious agents.
  • Follow local waste disposal rules.
  • Dilute below 103 copies/µL immediately before use.
  • Avoid freezing for low-copy dilutions to prevent DNA degradation.

INSTRUCTIONS FOR USE:

  • Open the foil pouch of AffiCHECK® Salmonella typhi DNA.
  • Centrifuge AffiCHECK® Salmonella typhi DNA Lyophilized Powder at 1000 g for 1 minute.
  • Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
  • The concentration will be 1x104 to 2x104 Copies/µL once reconstituted.
  • Vortex for 1 min for complete dissolution and homogenization.

RECOMMENDED STORAGE:

  • Before Reconstitution: Store at 2-8ºC.
  • After Reconstitution: Store at -20ºC.
  • Shipping: No special instructions
  • Shelf Life: 12 months from production date.

ORDERING INFORMATION :

Catalog Number Description Size
AFG-CHK-0557 AffiCHECK® Salmonella typhi DNA PCR Qualitative Positive Control 1 vial + 500 µL Buffer