AffiGEN® TransfectTM DNA In Vitro 5000
Product Description
Based on our proprietary polymer synthesis technology, AffiGEN® TransfectTM DNA In Vitro 5000 is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. The AffiGEN® Transfect 5000 is able to immobilize DNA migration during electrophoresis at very low concentration and form the transfection complex within 5 minutes at RT.
A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis , leading to much less cytotoxicity.
The reagent, 1.0 ml, is sufficient for more than 600 transfections in 24 well plates or more than 300 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.
Features
- Bio-degradable after endocytosis
- Exceptional high titers of virus production
- Equally good for very long DNAs (>89 kb)
- Equally good for both single DNA transfection and multi DNA co-transfection
- High levels of recombinant protein production
- Simple & robust transfection procedure
- Very affordable
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
DNA In Vitro Transfection Reagents Specifications
| Product | Nucleic Acid | Type | Delivery Method | Toxicity | Efficiency | Serum Tolerance |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | DNA | Calcium phosphate | Precipitation | High | High | High |
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | DNA, siRNA | Polymer | Polymer complex | Low | High | Low |
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | DNA, siRNA | Polymer | Polymer complex | Moderate | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | DNA | Polymer | Polymer complex | Low | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 3000 | DNA | Liposomal | Liposome-mediated | Moderate | High | High |
| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | DNA, siRNA | Liposomal | Liposome-mediated | Very low | High | High |
| AffiGEN® Transfect™ DNA In Vitro 5000 | DNA, siRNA | Polymer | Polymer complex | Moderate | High | High |
DNA In Vitro Transfection Reagents Application Guide
| Product | Applications | Cell Types | Cell lines | Suitable for | Additional Notes |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | Protein production, stable cell line generation, viral vector production | Adherent, some suspension | HEK293, HepG2, CHO | Large-scale transfection, cost-effective |
Lower calcium concentration than traditional methods
|
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Sensitive cell lines, maximizing cell viability |
Multiple formulations available
|
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Balancing efficiency and viability |
Released from DNA complex
|
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | Protein production, viral vector production | Most, including sensitive | Various | Long DNA, high efficiency |
Proprietary polymer
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| AffiGEN® Transfect™ DNA In Vitro 3000 | Viral vector production, antibody production, protein production | Adherent, suspension | Various | Lentivirus production, high efficiency |
Enhanced conjugate
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| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | Protein production, gene editing, virus production | Most, including sensitive | Various | Low toxicity, high efficiency |
Fluorinated cationic lipids
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| AffiGEN® Transfect™ DNA In Vitro 5000 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Common cell lines, biodegradable |
Biodegradable polycationic polymer
|