AffiVECTOR® pMXs-IRES-GFP Retroviral Vector
This vector offers high efficiency gene delivery with its AffiVECTOR® technology. It contains a pMXs backbone and IRES-GFP for easy tracking of target cells. Trust in our cutting-edge technology for successful gene modification and study.
AffiVECTOR® pMXs-IRES-GFP Retroviral Vector: Technical Applications
1. Gene Expression Studies
Internal Ribosome Entry Site (IRES): The pMXs-IRES-GFP vector incorporates an IRES element that allows for the co-expression of a gene of interest and GFP (Green Fluorescent Protein) from a single mRNA transcript. This ensures coordinated expression and is especially useful in experiments where consistent levels of both genes are required.
Reporter Gene: GFP serves as a fluorescent reporter, facilitating real-time visualization and quantification of gene expression in living cells. This helps in tracking the expression and localization of the gene of interest.
2. Retroviral Transduction
Stable Integration: As a retroviral vector, the pMXs-IRES-GFP integrates into the host genome, ensuring stable and long-term expression of the inserted genes. This is beneficial for studies that need persistent gene expression.
Transduction Efficiency: Retroviral vectors are highly efficient at transducing dividing cells. This makes the vector suitable for various cell types, particularly those that are proliferating, such as cancer cells and stem cells.
3. Cell Line Development
Selection Marker: The GFP gene acts as a selection marker for successfully transduced cells. Fluorescent microscopy or flow cytometry can be used to isolate GFP-positive cells, ensuring a population with high transduction efficiency.
Dual Expression: The IRES element allows for simultaneous expression of the gene of interest and GFP, enabling researchers to create cell lines that express the desired gene and can be easily identified by their fluorescence.
4. In Vivo Applications
Animal Models: The pMXs-IRES-GFP vector can be used to create transgenic animal models. GFP expression allows for the tracking and visualization of gene expression patterns in vivo, aiding in studies of developmental biology and disease mechanisms.
Tissue-specific Expression: By using tissue-specific promoters, researchers can achieve targeted expression of the gene of interest and GFP in specific tissues or cell types, which is crucial for studying tissue-specific gene functions and pathologies.
5. Drug Screening and Development
Assay Development: Stable cell lines expressing the gene of interest and GFP can be used in high-throughput screening assays. The fluorescent readout provided by GFP offers a straightforward and quantitative measure of assay outcomes, such as gene expression changes, cell viability, and responses to drug treatments.
Therapeutic Gene Validation: This vector can be used to validate the therapeutic potential of gene candidates. By observing the effects on GFP-expressing cells, researchers can identify promising therapeutic targets and understand their mechanisms of action.
6. CRISPR/Cas9 Genome Editing
Co-expression of CRISPR Components: The IRES element allows for the co-expression of CRISPR/Cas9 components and GFP. This facilitates the creation of stable cell lines for genome editing studies, ensuring efficient and uniform expression of the necessary components.
Selection of Edited Cells: GFP expression serves as a marker to identify and sort successfully edited cells following CRISPR/Cas9-mediated genome editing. This streamlines the process of identifying and expanding edited cell populations.
7. Stem Cell Research
Stable Gene Expression: The pMXs-IRES-GFP vector is useful for studying gene function in stem cells by providing stable expression of the gene of interest. This is crucial for long-term studies on stem cell differentiation, proliferation, and lineage tracing.
Selection and Tracking: GFP expression allows for the efficient selection and tracking of transduced stem cells. This is valuable for understanding stem cell behavior and fate during differentiation and in various experimental conditions.
8. Oncogene and Tumor Suppressor Studies
Cancer Research: This vector can be used to study the roles of oncogenes and tumor suppressor genes by co-expressing these genes with GFP. Researchers can investigate the effects on cell proliferation, transformation, and tumorigenesis.
Tumor Marker: GFP expression in cancer cells serves as a tumor marker, enabling the study of tumor growth, metastasis, and response to therapeutic interventions in vivo.
The AffiVECTOR® pMXs-IRES-GFP Retroviral Vector is a powerful and versatile tool for molecular biology and biomedical research. Its applications span gene expression studies, cell line development, in vivo research, drug screening, CRISPR/Cas9 genome editing, stem cell research, and cancer studies. The vector's ability to provide stable and coordinated expression of a gene of interest and GFP, combined with the benefits of retroviral transduction, make it an indispensable resource for a wide range of advanced research applications.