AffiPURE® Cell/Tissue DNA Isolation Mini Kit
AffiPURE® Cell/Tissue DNA Isolation Mini Kit is intended for the extraction of genomic DNA from 1 - 20 mg of animal tissues and less than 5 × 10^6 cultured cells. This kit is based on silica gel column purification technology that eliminates the need for extraction using phenol/chloroform organic solvents or time-consuming alcohol precipitation. The whole extraction process takes only 30 - 60 min. The obtained DNA is ready for use in enzyme digestion, PCR, library preparation, virus DNA detection, etc.
Components:
| Components | AFG-VZ-DC102-01 |
| Buffer GA | 40 mL |
| Buffer GB | 40 mL |
| RNase Solution | 1,1 mL |
| Proteinase K | 2 mL |
| Washing Buffer A | 26 mL |
| Washing Buffer B | 40 mL |
| Elution Buffer | 40 mL |
| AffiPURE gDNA Columns | 100 |
| Collection Tubes 2 ml | 100 |
Notes:
Buffer GA: Provide an environment for sample lysis.RNase Solution: Degrade RNA in the sample.
Washing Buffer A: Remove proteins and other impurities from genomic DNA.
Elution Buffer: Elute genomic DNA from the spin column.
Buffer GB: Elute Proteinase K and provides an environment for binding.
Proteinase K: Lyse tissue sample.
Washing Buffer B: Remove salt ions from genomic DNA.
AffiPURE gDNA Columns: Adsorb genomic DNA.
Collection Tubes 2 mL: Collect filtrate
Storage:
Store Proteinase K and RNase Solution at -30 ~ -15°C and transport at room temperature.Store the other components at 15 ~ 25°C and transport at room temperature.
Applications:
1 - 20 mg of animal tissues.<5 × 10^6 cultured cells.
Liquid samples.
