AffiPCR® All-in-one RT SuperMix Perfect for qPCR V3
AffiPCR® All-in-one RT SuperMix Perfect for qPCR V3 is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper). Within one step, genomic removal and reverse transcription reactions can be achieved simultaneously. The simpler and faster opereation ensure reduce risk of sample contamination and RNA degradation. This product contains HiScript III Reverse Transcriptase, heat-labile DNase and an optimized buffer system. The heat-labile DNase works quickly and efficiently; and it could be inactivated easily. The RT product is compatible with dye-based and probe-based qPCR, and can perform high-performance gene expression analysis.
Storage:
Store at -30 ~ -15°C and transport at ≤0°C.
Application:
It is applicable for reverse transcription reaction of animal, plant and microbial RNA. The RT product is compatible with dye-based and probe-based qPCR.
Components:
| Component | Quantity |
| RNase-free ddH2O | 2 × 1 mL |
| Enzyme Mix* | 100 μL |
| 5X All-in-one qRT SuperMix* | 400 μL |
| No RT Control Mix* | 20 μL |
Equivalence:
| AFG-VZ-R333 | AffiGEN AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V3 |
| RR047 Q/A/4XA | TaKaRa PrimeScrip™ RT reagent Kit with gDNA Eraser (Perfect Real Time) |