AffiPCR® AceTaq DNA Polymerase

AffiPCR® AceTaq DNA Polymerase

AffiPCR® AceTaq DNA Polymerase is a chemically modified Taq DNA Polymerase that is completely blocked at room temperature and is released only after heating at 95℃. Non-specific amplification and primer dimerization can be prevented during sample preparation and temperature rise. Compared with the antibody-based hot-start Taq, the polymerase activity of AceTaq DNA Polymerase is blocked more stringently and completely. It takes only 5 min to activate AceTaq DNA Polymerase. AceTaq DNA Polymerase is compatible with most existing PCR protocols. Combined with an optimized buffer system, AceTaq DNA Polymerase minimizes non-specific amplification and primer dimers, ensuring extremely high sensitivity and specificity, which make it ideal for amplifying low-copy genes from complex templates. The PCR products contain A at the 3' end and can be directly cloned into T-Vector. The products are compatible with ClonExpress and Top Cloning kits.

Storage Conditions:

Store at -30°C ~ -15°C and transport at ≤0°C.

Applications:

This product is suitable for DNA amplification from various type of templates such as genomic DNA, cDNA, plasmid DNA and λDNA.

Notes:

For research use only. Not for use in diagnostic procedures.

Unit Definition:

One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTP into acid-insoluble material in 30 min at 74°C with activated salmon sperm DNA as the template/primer.