AffiGEN® TransfectTM siRNA In Vitro 2000 Buffer (5X)
Our Enhanced AffiGEN® TransfectTM siRNA In Vitro 2000 Buffer (5X) has been specially formulated to ensure the highest level of transfection efficiency and optimal gene expression knockdown when used in conjunction with our AffiGEN® TransfectTM siRNA In Vitro 2000. This advanced buffer is designed for diluting siRNA/DNA and our AffiGEN® TransfectTM siRNA In Vitro 2000, resulting in the formation of the most efficient transfection complex possible.
Product Details:
Product Name: AffiGEN® TransfectTM siRNA In Vitro 2000 Buffer (5X)
Size: 8.0 mL/vial, to prepare 40 mL of working solution (1X concentration)
Sufficient for 1000 transfections in a 24-well plate.
Storage:
Store at room temperature (RT).
When stored properly, this product maintains stability for at least 12 months or longer.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |