SKU: AFG-CRB-4690

AffiBEADS® Streptavidin Magnetic Beads

Vendor AffiGEN
Regular price 1.849,00 DKK
Sale price 1.849,00 DKK Regular price
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AffiBEADS® Streptavidin Magnetic Beads

AffiBEADS® Streptavidin Magnetic Beads are designed for high-efficiency binding with biotinylated molecules, facilitating various applications such as immunoprecipitation, protein purification, and cell isolation. These beads exhibit strong magnetic properties, ensuring rapid and efficient magnetic separation.

AffiBEADS® Streptavidin Magnetic Beads Features

  • High Binding Capacity: Efficient binding with biotinylated molecules.
  • Versatile Applications: Ideal for immunoprecipitation, protein purification, and cell isolation.
  • Rapid Separation: Strong magnetic properties for quick and efficient separation.

AffiBEADS® Streptavidin Magnetic Beads Specifications

  • Bead Diameter: 2.8 µm.
  • Binding Capacity: High affinity and specificity for biotin.
  • Storage Conditions: Store at 2-8°C, avoid freezing.

AffiBEADS® Streptavidin Magnetic Beads Applications

  • Immunoprecipitation
  • Protein Purification
  • Cell Isolation

AffiBEADS® Streptavidin Magnetic Beads Protocol

Prepare Beads:

  • Reconstitute the beads in ultrapure water to 1 mg/mL.
  • Wash the beads three times.

Bind Proteins:

  • Dilute the biotinylated protein or antibodies with Assay Buffer.
  • Add to the beads and incubate for 60 minutes at room temperature on a rotary mixer.

Final Wash:

  • Wash the beads four times.
  • Add Assay Buffer to the beads for the next experiment.

Note:

Streptavidin has an extremely high affinity for biotin, making the binding nearly irreversible. These beads are pre-coupled with Streptavidin and blocked with BSA, ready for immediate use.