AffiASSAY® Citrate Colorimetric & Fluorometric Assay Kit

AffiASSAY® Citrate Colorimetric & Fluorometric Assay Kit

AffiASSAY® Citrate Colorimetric & Fluorometric Assay Kit is designed to provide precise and reliable measurements of citrate levels in biological samples. This assay kit offers both colorimetric and fluorometric detection methods, making it a versatile tool for researchers in biochemistry, physiology, and nutrition.

Key Features:

• High Sensitivity: Our assay kit is optimized to detect citrate levels with high sensitivity, allowing for accurate quantification even in low concentration samples.
• Colorimetric & Fluorometric Detection: With the flexibility to choose between colorimetric or fluorometric detection methods, our kit provides options for different experimental setups and preferences.
• Wide Application: The AffiASSAY® Citrate Assay Kit can be used with various sample types, including serum, plasma, urine, tissue homogenates, and cell lysates.
• Quick and Easy: With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.
• Versatile: Whether you're studying citrate metabolism, investigating its role in metabolic diseases, or assessing dietary intake, our kit provides the versatility you need.

Assay Principle:

This assay kit measures citrate levels based on the enzymatic reactions involving citrate. Citrate is metabolized to produce a product that reacts with a colorimetric or fluorometric probe to generate a signal proportional to citrate concentration.

Applications:

• Metabolic Research: Study citrate metabolism and its involvement in various metabolic pathways, including the citric acid cycle.
• Nutrition Studies: Assess dietary intake of citrate and its correlation with health outcomes.
• Medical Diagnostics: Measure citrate levels as potential biomarkers for metabolic disorders such as diabetes or metabolic syndrome.

Kit Contents:

• Citrate Assay Buffer
• Citrate Enzyme Mix
• Citrate Probe
• 96-Well Assay Plate
• Detailed Protocol

Why Choose AffiASSAY®?

Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.

AffiASSAY® Citrate Colorimetric & Fluorometric Assay Kit Protocol:

[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]

Materials Needed:

• AffiASSAY® Citrate Colorimetric & Fluorometric Assay Kit
• Biological samples containing citrate
• 96-well microplate
• Microplate reader capable of measuring absorbance or fluorescence intensity
• Pipettes and tips
• Microcentrifuge tubes

Protocol:

Standard Curve Preparation:

• Prepare a series of standard solutions containing known concentrations of citrate.
• Dilute the Citrate Probe provided in the kit with the Assay Buffer to prepare the working solution.
• Add the working solution to each standard solution to initiate the reaction.

Assay Plate Setup:

• Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
• If using controls, prepare control samples without citrate to serve as background controls.

Sample and Standard Addition:

• Add 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.
• Ensure that the final reaction volume in each well is consistent.

Assay Reaction:

• Add 50 µL of Citrate Enzyme Mix to each well to initiate the reaction.
• Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.

Signal Detection:

• For colorimetric detection: Measure the absorbance at the appropriate wavelength using a microplate reader.
• For fluorometric detection: Measure the fluorescence intensity using a microplate reader with excitation and emission wavelengths suitable for the probe provided in the kit.

Data Analysis:

• Generate a standard curve using the absorbance or fluorescence values obtained from the standard solutions.
• Calculate the citrate concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curve.

Results Interpretation:

• Interpret and analyze your results according to your research objectives.
• Note any variations or trends in citrate levels among your samples.

Data Presentation:

• Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.

Cleanup:

• Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
• Always refer to the specific instructions provided with the AffiASSAY® Citrate Colorimetric & Fluorometric Assay Kit for any kit-specific details or variations in the protocol.
• Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.