AffiASSAY® Calcium Colorimetric Assay Kit
AffiASSAY® Calcium Colorimetric Assay Kit is designed to provide precise and reliable measurements of calcium levels in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of physiology, cell biology, and calcium signaling.
Key Features:
- High Sensitivity: Our assay kit is optimized to detect even low levels of calcium, allowing for accurate quantification in a wide range of samples.
- Colorimetric Detection: Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.
- Wide Application: The AffiASSAY® Calcium Colorimetric Assay Kit can be used with various sample types, including serum, plasma, cell lysates, tissue homogenates, and culture supernatants.
- Quick and Easy: With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.
- Versatile: Whether you're studying calcium homeostasis, investigating calcium-dependent signaling pathways, or evaluating the effects of calcium-modulating agents, our kit provides the versatility you need.
Assay Principle:
This assay kit employs a colorimetric method based on the reaction between calcium ions and a chromogenic reagent. The formation of a colored complex is directly proportional to the concentration of calcium in the sample, allowing for precise quantification.
Applications:
- Cellular Physiology: Measure calcium levels in cell lysates or culture supernatants to assess intracellular calcium signaling and calcium-dependent processes.
- Medical Research: Investigate the role of calcium in various physiological processes, such as muscle contraction, neurotransmitter release, and enzyme activation.
- Drug Discovery: Screen compounds for their effects on calcium levels and calcium-dependent pathways, aiding in the development of novel therapeutics for diseases associated with calcium dysregulation.
Kit Contents:
- Assay Buffer
- Calcium Chromogenic Reagent
- Calcium Standard
- 96-Well Assay Plate
- Detailed Protocol
Why Choose AffiASSAY®?
Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.
AffiASSAY® Calcium Colorimetric Assay Kit Protocol:
[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]
Materials Needed:
- AffiASSAY® Calcium Colorimetric Assay Kit
- Biological samples containing calcium
- 96-well microplate
- Microplate reader capable of measuring absorbance at specific wavelengths
- Pipettes and tips
- Microcentrifuge tubes
Protocol:
Standard Curve Preparation:
- Reconstitute the Calcium Standard provided in the kit according to the kit's instructions.
- Create a series of standard dilutions using the reconstituted Calcium Standard to generate a standard curve. Typical concentrations may range from 0 to 10 mM.
Assay Plate Setup:
- Label the microplate wells according to your experimental design, including wells for your samples, standards, and blanks.
- Prepare blank wells containing assay buffer only.
Sample and Standard Addition:
- Add 50-100 µL of your prepared samples and Calcium Standards to the appropriate wells on the microplate.
- Ensure that the final reaction volume in each well is consistent.
Calcium Reaction:
- Add the Calcium Chromogenic Reagent to each well to initiate the reaction.
- Mix the contents gently and incubate the microplate at room temperature for a specified time to allow color development.
Measurement:
- Measure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.
Data Analysis:
- Generate a standard curve using the absorbance values obtained from the Calcium Standards.
- Calculate the calcium concentrations in your samples based on their respective absorbance readings and the standard curve.
Results Interpretation:
- Interpret and analyze your results according to your research objectives.
- Note any variations or trends in calcium levels among your samples.
Data Presentation:
- Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.
Cleanup:
- Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
- Always refer to the specific instructions provided with the AffiASSAY® Calcium Colorimetric Assay Kit for any kit-specific details or variations in the protocol.
- Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.
