AffiNGS® VAHTS Universal Plus Fragmentation,End Preparation & dA-Tailing Module for Illumina V2
AffiNGS® VAHTS Universal Plus Fragmentation,End Preparation & dA-Tailing Module for Illumina V2 is developed specifically for Illumina high-throughput sequencing platform, support fragmentation, end-repair and dA-tailing ina single reaction step.The module offerts improved fragmentation performance, which significantly reduces the proportion of Artificial Invert Chimera Reads in the FFPE sample DNA library and improves the reliability of SNV detection. The module has been optimized to convert 100 pg - 1 µg of DNA to fragmented, end-repaired DNA having 5' phosphorylated, 3' dA-tailed ends and qualified with automation methods, greatly reducing the total operation time. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.
Components:
| Components |
AFG-VZ-N219-01 (24 rxns) |
AFG-VZ-N219-02 (96 rxns) |
| FEA Buffer V2 | 120 µl | 480 µl |
| FEA Enzyme Mix V2 | 240 µl | 960 µl |
| Neutralization Buffer | 120 µl | 480 µl |
Storage:
Store at -30 ~-15°C and transport at ≤0° C.