AffiPCR® miRNA 1st Strand cDNA Synthesis Kit (by tailing A)
AffiPCR® miRNA 1st strand cDNA Synthesis Kit (by tailing A) is a kit for microRNA (miRNA) reverse transcription (RT) by tailing A. Poly A polymerase (PAP) adds a Poly(A) tail to the 3' end of miRNA or total RNA, and then universal reverse transcription primers initiate RT reaction with reverse transcriptase. HiScript miRNA Enzyme Mix contains bioengineered reverse transcriptase and PAP. Combined with the optimized buffer, the reverse transcription of non-miRNAs is effectively inhibited, achieving highly specific qPCR results. 2X miRNA RT Mix and HiScript miRNA Enzyme Mix contains all components required for miRNA tailing and reverse transcription. Two reactions can be performed simultaneously in one tube.
Components:
| Components | 20 Reactions | 50 Reactions |
| RNase-free ddH2O | 1 mL | 1 mL |
| 2X miRNA RT Mixa | 200 μL | 500 μL |
| HiScript miRNA Enzyme Mix | 40 μL | 100 μL |
| Universal reverse Q primerc (10 μM) | 500 μL | 1.25 mL |
Applications:
It is applicable for A-tailed reverse transcription using total RNA or miRNA as a template.
Notes:
For research use only. Not for use in diagnostic procedures.
- Prevent RNase contamination Please keep the experiment area clean; Wear disposable gloves and masks; Use RNase-free consumables such as centrifuge tubes and pipette tips.
- Prepare the mixture on ice.
Storage Conditions:
Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.