AffiNGS® Hyperactive pG-MNase CUT&RUN Assay Kit for Illumina
The AffiNGS® Hyperactive pG-MNase CUT&RUN Assay Kit for Illumina is a reagent kit designed for studying protein-DNA interactions, specifically tailored for the Illumina high-throughput sequencing platform. The CUT&RUN (Cleavage Under Targets and Release Using Nuclease) technique is a novel method for investigating protein-DNA interactions. This method utilizes MNase (Micrococcal Nuclease) fused with Protein G, which enables precise targeting of specific proteins guided by antibodies. The MNase enzyme cleaves DNA fragments in proximity to the target sites. This assay kit has been optimized for experimental conditions and library preparation workflows.
Compared to traditional ChIP-Seq methods, the kit offers several advantages such as high experimental success rate, strong antibody compatibility, shorter experimental time, and simplified procedures. It is particularly well-suited for research areas such as early embryonic development, stem cells, oncology, and epigenetics. All reagents included in the kit undergo stringent quality control and functional validation, ensuring the stability and reproducibility of library construction.
Components:
| Components |
AFG-VZ-HD102-01 (24 rxns) |
|
| BOX 1 | ConA Beads Pro | 260 µl |
| BOX 2 | FastPure gDNA Mini Columns | 24 ml |
| Collection Tubes 2 ml | 24 ml | |
| Buffer GDP | 24 ml | |
| Buffer GW | 4 ml | |
| BOX 3 | pG-MNase Enzyme | 30 µl |
| MNase Dilution Buffer | 2 x 1,3 ml | |
| 5% Digitonin | 1,3 ml | |
| 10 × Binding Buffer | 800 µl | |
| 10 × Wash Buffer | 12 ml | |
| Antibody Buffer(-) | 2 x 1,2 ml | |
| CaCl2 | 48 µl | |
| 2 × Stop Buffer(-) | 2 x 1,2 ml | |
| Spike in DNA (5 ng/μl) | 24 µl | |
| DNA Damage Repair Enzyme | 48 µl | |
| End Prep Buffer | 240 µl | |
| End Prep Enzyme | 120 µl | |
| Rapid Ligation Buffer | 600 µl | |
| Rapid DNA Ligase | 120 µl | |
| VAHTS HiFi Amplification Mix | 600 µl | |
| PCR Primer Mix3 for Illumina | 120 µl | |
Storage:
BOX 1: ConA Beads Pro, store at 2 ~ 8℃, adjust shipping method based on destination; BOX 2: Store at 15 ~ 25℃, ship at room temperature; BOX 3: 5% Digitonin, store at -30 ~ -15℃, can be stored at room temperature (15 ~ 25℃) for 1 week; Other components, store at -30 ~ -15℃; Transport at or below ≤0℃.