AffiAB® Goat Anti-RFP, DyLight® 405 IgG Antibody is an optimally formulated research reagent specifically designed for the detection of RFP. It provides over 95% specificity and cross reactivity, while remaining highly sensitive. The DyLight® 405 conjugation ensures maximum selectivity and accuracy in fluorescent detection.
The AffiAB® Goat Anti-RFP, DyLight® 405 Polyclonal IgG Antibody is an antibody specifically designed to target and bind to RFP (Red Fluorescent Protein) when conjugated with DyLight® 405, a blue-fluorescent dye. This antibody is produced by immunizing goats with RFP protein or peptides, and the resulting polyclonal antibody is purified and conjugated with the DyLight® 405 dye.
RFP is a widely used fluorescent protein derived from Discosoma sp. coral, which emits red fluorescence when excited by appropriate wavelengths of light. By conjugating the anti-RFP antibody with DyLight® 405, the resulting antibody can be excited by blue light and emit a blue fluorescence signal when bound to RFP-tagged proteins.
The AffiAB® Goat Anti-RFP, DyLight® 405 Polyclonal IgG Antibody is commonly used in fluorescence-based applications such as immunofluorescence, immunocytochemistry, and flow cytometry. It allows researchers to specifically detect and visualize RFP-tagged proteins in biological samples using blue excitation and detection channels.
When using this antibody, it is important to follow the recommended protocols provided by the manufacturer for sample preparation, antibody dilution, and incubation times. Proper controls and optimization steps should also be performed to ensure specific binding and minimize background signals.
In summary, the AffiAB® Goat Anti-RFP, DyLight® 405 Polyclonal IgG Antibody is a valuable tool for researchers working with RFP-tagged proteins who require a blue-fluorescent detection system. It enables the specific visualization and analysis of RFP-tagged proteins in various experimental setups, contributing to the understanding of cellular processes and protein dynamics.