SKU: AFG-M-0162

AffiZYME® T4 DNA polymerase

Vendor AffiGEN
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AffiZYME® T4 DNA polymerase

AffiZYME® T4 DNA Polymerase catalyzes DNA synthesis in the 5'---->3' direction in the presence templates and primers. This enzyme also has 3'---->5' exonuclease activity which is more active than that of DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase has no 5'---->3' exonuclease activity. 

Components:

Components AFG-VZ-N101-01
2,000 U
T4 DNA polymerase (3 U/μL) 667 μL
10X Blue Buffer 2 x 1 mL

 

Reaction Buffer:

10X Blue Buffer:

100 mM Tris-HCI pH 7.9 at 25°C 500 mM NaCl
100 mM MgCb
10 mM DTT

Storage:

Store at -30°C to -15°C and transport at ≤0°C.

Applications:

Removal of 3' overhangs or fill-in of 5' overhangs to form blunt ends.

Source:

Recombinant E.coli strains carrying the cloned T4 bacteriophage DNA polymerase gene.

Unit Definition:

One unit of activity (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble precipitates within 30 min at 37°C.

Notes:

For research use only. Not for use in diagnostic procedures.

It is recommended to place the enzyme on ice during use and put it back to -20°C immediately after use .

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