AffiZYME® T4 DNA polymerase
AffiZYME® T4 DNA Polymerase catalyzes DNA synthesis in the 5'---->3' direction in the presence templates and primers. This enzyme also has 3'---->5' exonuclease activity which is more active than that of DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase has no 5'---->3' exonuclease activity.
Components:
| Components |
AFG-VZ-N101-01 2,000 U |
| T4 DNA polymerase (3 U/μL) | 667 μL |
| 10X Blue Buffer | 2 x 1 mL |
Reaction Buffer:
10X Blue Buffer:
100 mM Tris-HCI pH 7.9 at 25°C 500 mM NaCl
100 mM MgCb
10 mM DTT
Storage:
Store at -30°C to -15°C and transport at ≤0°C.
Applications:
Removal of 3' overhangs or fill-in of 5' overhangs to form blunt ends.
Source:
Recombinant E.coli strains carrying the cloned T4 bacteriophage DNA polymerase gene.
Unit Definition:
One unit of activity (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble precipitates within 30 min at 37°C.
Notes:
For research use only. Not for use in diagnostic procedures.
It is recommended to place the enzyme on ice during use and put it back to -20°C immediately after use .