AffiZOL® Lysis Reagent

AffiZOL® Lysis Reagent
(Phenol-Guanidine Solution for RNA, DNA & Protein Extraction)

Product Description

AffiZOL® Lysis Reagent is a monophasic phenol and guanidine isothiocyanate solution optimized for the rapid extraction of high-quality total RNA, with optional recovery of DNA and proteins from the same sample. Its robust performance across diverse sample types makes it ideal for high-throughput RNA workflows in molecular biology, diagnostics, and functional genomics.

AffiZOL® effectively disrupts cells, dissolves cell components, and simultaneously inactivates RNases and DNases—ensuring RNA integrity during the entire extraction process.

Features & Benefits

• 🔬 Three-in-One Extraction – Isolate RNA, DNA, and proteins from a single sample

• ⚡ Fast & Convenient Protocol – Minimal hands-on time with consistent yields

• 🧪 Superior RNA Integrity – Ideal for downstream applications like RT-qPCR, RNA-seq, and microarrays

• 🌱 Broad Sample Compatibility – Efficient on tissues, cultured cells, blood, bacteria, and plant material

• 🧊 Stable & Ready-to-Use – Long shelf life when stored at 2–8°C

Applications

• Total RNA extraction for:

  • RT-PCR / qPCR

  • RNA-Seq

  • Northern blotting

  • Gene expression analysis

• Genomic DNA and protein co-extraction

• Sample preparation for multi-omics workflows

Protocol Overview

1. Homogenize sample in AffiZOL® Reagent

2. Add chloroform and centrifuge to separate phases

3. Recover RNA from aqueous phase

4. Optionally extract DNA and proteins from interphase and organic phase

Storage & Stability

• Store at 2–8°C, protected from light

• Shelf life: 12 months