AffiPCR® 1st Strand cDNA Synthesis Kit V2
AffiPCR® HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology based on M-MLV (RNase H-) Reverse Transcriptase. Compared with HiScript Reverse Transcriptase, HiScript II further improves the thermal stability and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript II adds several point mutations that further enhances template binding affinity and cDNA synthesis efficiency, and has higher tolerance to common reverse transcription inhibitors. This product is 1st strand cDNA synthesis kit based on HiScript II Reverse Transcriptase. It contains all the components necessary for high quality first strand cDNA synthesis, and the product is suitable for subsequent PCR, qPCR and other experiments. The 2X RT Mix contains an optimized buffer and dNTPs. The HiScript II Enzyme Mix contains the HiScript II Reverse Transcriptase and the RNase inhibitor. Oligo (dT)23VN has stronger anchoring ability for poly A+ mRNA than Oligo (dT)18, which makes reverse transcription more efficient. Oligo (dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. The kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.
Components:
| Components | 50 Reactions | 100 Reactions |
| RNase-free ddH2O | 1 mL | 1 mL |
| 2X RT Mixa | 500 μL | 1 mL |
| HiScript II Enzyme Mixb | 100 μL | 200 μL |
| Oligo (dT)23VN (50 μM) | 50 μL | 100 μL |
| Random hexamers (50 ng/μl) | 50 μL | 100 μL |
Storage Conditions:
Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.
Equivalence:
| AFG-VZ-R211 | AffiGEN AffiPCR® 1st Strand cDNA Synthesis Kit V2 |
| FSK-101 | TOYOBO ReverTra Ace -ɑ-® |
| E6560 | NEB ProtoScript® II First Strand cDNA Synthesis Kit |
| RR037 | TaKaRa PrimeScript™ RT reagent Kit (Perfect Real Time) |