Load image into Gallery viewer, AffiGEN® Transfect(TM) DNA In Vitro 2000 V1
Load image into Gallery viewer, AffiGEN® Transfect(TM) DNA In Vitro 2000 V1
Load image into Gallery viewer, AffiGEN® Transfect(TM) DNA In Vitro 2000 V1

SKU: AFG-SNG-TR-003

AffiGEN® Transfect(TM) DNA In Vitro 2000 V1

Vendor AffiGEN
Regular price CHF 177.00
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AffiGEN® TransfectTM DNA In Vitro 2000 V1

Product Description

AffiGEN® TransfectTM DNA In Vitro 2000 V1 is a potent transfection reagent that ensures highly efficient and consistent transfection outcomes while maintaining minimal toxicity. It utilizes a unique chemical formulation involving the covalent cross-linking of cationic lipids with polymers, resulting in remarkable transfection efficiency and distinct advantages over other types of transfection reagents. AffiGEN® TransfectTM DNA In Vitro 2000 V1 has demonstrated its ability to successfully deliver genes to a diverse range of cell lines, both established and primary, including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines such as Sf9 and Sf21, and various other eukaryotic cell lines. One unit of AffiGEN® TransfectTM DNA In Vitro 2000 V1, with a volume of 1.0 ml, is suitable for more than 600 transfections in 24-well plates or around 333 transfections in 6-well plates.

Application

 - Facilitates the delivery of DNA/RNA into mammalian and insect cells.
 - Dramatically enhances virus infection efficiency (up to 200 times) for challenging-to-transfect cells, including replication-deficient Adenovirus.

Features

 - Exceptionally high virus production yields.
 - Equally effective for very long DNA molecules (up to 56 kb).
 - Suitable for both single and multiple DNA transfections.
 - Achieves exceptionally high levels of recombinant protein production.
 - The preferred choice for challenging-to-transfect cells.
 - A cost-effective transfection reagent.

Storage Condition

Store AffiGEN® TransfectTM DNA In Vitro 2000 V1 at 4°C. When stored correctly, the product maintains stability for 12 months or longer.

 

DNA In Vitro Transfection Reagents Specifications

Product Nucleic Acid Type Delivery Method Toxicity Efficiency Serum Tolerance
AffiGEN® Transfect™ 1000 - Mammalian Cell DNA Calcium phosphate Precipitation High High High
AffiGEN® Transfect™ DNA In Vitro 2000 V1 DNA, siRNA Polymer Polymer complex Low High Low
AffiGEN® Transfect™ DNA In Vitro 2000 V2 DNA, siRNA Polymer Polymer complex Moderate Higher Medium
AffiGEN® Transfect™ DNA In Vitro 2000 Plus DNA Polymer Polymer complex Low Higher Medium
AffiGEN® Transfect™ DNA In Vitro 3000 DNA Liposomal Liposome-mediated Moderate High High
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit DNA, siRNA Liposomal Liposome-mediated Very low High High
AffiGEN® Transfect™ DNA In Vitro 5000 DNA, siRNA Polymer Polymer complex Moderate High High

 

DNA In Vitro Transfection Reagents Application Guide

Product Applications Cell Types Cell lines Suitable for Additional Notes
AffiGEN® Transfect™ 1000 - Mammalian Cell Protein production, stable cell line generation, viral vector production Adherent, some suspension HEK293, HepG2, CHO Large-scale transfection, cost-effective
Lower calcium concentration than traditional methods
AffiGEN® Transfect™ DNA In Vitro 2000 V1 Gene expression studies, protein production, virus production Most, including sensitive Various Sensitive cell lines, maximizing cell viability
Multiple formulations available
AffiGEN® Transfect™ DNA In Vitro 2000 V2 Gene expression studies, protein production, virus production Most, including sensitive Various Balancing efficiency and viability
Released from DNA complex
AffiGEN® Transfect™ DNA In Vitro 2000 Plus Protein production, viral vector production Most, including sensitive Various Long DNA, high efficiency
Proprietary polymer
AffiGEN® Transfect™ DNA In Vitro 3000 Viral vector production, antibody production, protein production Adherent, suspension Various Lentivirus production, high efficiency
Enhanced conjugate
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit Protein production, gene editing, virus production Most, including sensitive Various Low toxicity, high efficiency
Fluorinated cationic lipids
AffiGEN® Transfect™ DNA In Vitro 5000 Gene expression studies, protein production, virus production Most, including sensitive Various Common cell lines, biodegradable
Biodegradable polycationic polymer