Overview
Annexin V-FITC/PI Apoptosis Detection Kit uses FITC-labeled Annexin V as a probe to detect early apoptosis of cells. The detection principle is that in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS reverses from the inner side to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V is a Ca2+ -dependent phospholipid binding protein with a molecular weight of 35-36 kDa. Annexin V is a Ca2+ -dependent phospholipid binding protein with a high affinity for PS and binds to the membranes of early-apoptotic cells via externally exposed phosphatidylserine. In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-) . The apoptotic cells and necrotic cells were double positive by FITC and PI binding staining (Annexin V+/PI+) . This kit can be used for flow cytometry and fluorescence microscopy.
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