AffiASSAY® D-Lactate Colorimetric Assay Kit
The AffiASSAY® D-Lactate Colorimetric Assay Kit provides a precise and reliable method for measuring D-lactate levels in various biological samples. This assay kit offers a simple and efficient solution for researchers in fields such as clinical diagnostics, microbiology, and nutrition.
Key Features:
- High Sensitivity: Our assay kit can detect low levels of D-lactate, ensuring accurate quantification in biological samples.
- Colorimetric Detection: Utilizing a colorimetric assay, our kit offers a simple and easy-to-read readout, suitable for both manual and automated analysis.
- Wide Application: The AffiASSAY® D-Lactate Assay Kit is compatible with various sample types, including serum, plasma, urine, tissue homogenates, and cell lysates.
- Quick and Easy: With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.
- Cost-Effective: Our kit provides an affordable solution for measuring D-lactate levels, reducing experimental costs without compromising data quality.
Assay Principle:
This assay kit measures D-lactate levels based on the enzymatic conversion of D-lactate to generate a product that reacts with a colorimetric probe. The intensity of the color formed is proportional to the D-lactate concentration in the sample.
Applications:
- Clinical Diagnostics: Measure D-lactate levels in patient samples for the diagnosis and monitoring of lactate acidosis, bacterial infections, and other metabolic disorders.
- Microbiology: Assess D-lactate production by bacteria and other microorganisms in culture media or biological samples.
- Nutrition Studies: Evaluate D-lactate levels in food products and beverages to assess fermentation processes and food quality.
Kit Contents:
- D-Lactate Assay Buffer
- D-Lactate Enzyme Mix
- D-Lactate Probe
- 96-Well Assay Plate
- Detailed Protocol
Why Choose AffiASSAY®?
Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.
AffiASSAY® D-Lactate Colorimetric Assay Kit Protocol:
[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]
Materials Needed:
- AffiASSAY® D-Lactate Colorimetric Assay Kit
- Biological samples containing D-lactate
- 96-well microplate
- Microplate reader capable of measuring absorbance
- Pipettes and tips
- Microcentrifuge tubes
Protocol:
Standard Curve Preparation:
- Prepare a series of standard solutions containing known concentrations of D-lactate.
- Dilute the D-Lactate Probe provided in the kit with the Assay Buffer to prepare the working solution.
- Add the working solution to each standard solution to initiate the reaction.
Assay Plate Setup:
- Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
- If using controls, prepare control samples without D-lactate to serve as background controls.
Sample and Standard Addition:
- Add 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.
- Ensure that the final reaction volume in each well is consistent.
Assay Reaction:
- Add 50 µL of D-Lactate Enzyme Mix to each well to initiate the reaction.
- Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.
Colorimetric Measurement:
- After the incubation period, measure the absorbance of each well at the appropriate wavelength using a microplate reader.
Data Analysis:
- Generate a standard curve using the absorbance values obtained from the standard solutions.
- Calculate the D-lactate concentrations in your samples based on their respective absorbance readings and the standard curve.
Results Interpretation:
- Interpret and analyze your results according to your research objectives.
- Observe any variations or trends in D-lactate levels among your samples.
Data Presentation:
- Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.
Cleanup:
- Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
- Always refer to the specific instructions provided with the AffiASSAY® D-Lactate Colorimetric Assay Kit for any kit-specific details or variations in the protocol.
- Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.
