AffiASSAY® Catalase Activity Colorimetric Assay Kit

AffiASSAY® Catalase Activity Colorimetric Assay Kit

AffiASSAY® Catalase Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of catalase enzyme activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and oxidative stress research.

Key Features:

• High Sensitivity: Our assay kit is optimized to detect catalase activity, allowing for accurate quantification in a wide range of samples.
• Colorimetric Detection: Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.
• Wide Application: The AffiASSAY® Catalase Activity Colorimetric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, blood, serum, plasma, and culture supernatants.
• Quick and Easy: With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.
• Versatile: Whether you're studying oxidative stress, assessing antioxidant enzyme activity, or investigating the effects of drug compounds, our kit provides the versatility you need.

Assay Principle:

This assay kit measures catalase activity based on the decomposition of hydrogen peroxide (H₂O₂) by catalase enzyme. The reaction produces water and oxygen, and the decrease in H₂O₂ concentration is monitored by the decrease in absorbance at a specific wavelength, allowing for the quantification of catalase activity.

Applications:

• Oxidative Stress Research: Assess catalase activity as part of oxidative stress studies to understand the balance between reactive oxygen species (ROS) production and antioxidant defense mechanisms.
• Cell Biology: Measure catalase activity in cell lysates or culture supernatants to evaluate cellular antioxidant capacity and response to oxidative insults.
• Biomedical Research: Investigate the role of catalase in various physiological processes, including inflammation, aging, and disease states such as cancer and neurodegenerative disorders.

Kit Contents:

• Assay Buffer
• Catalase Substrate (Hydrogen Peroxide)
• Catalase Standard
• Potassium Hydroxide (KOH)
• 96-Well Assay Plate
• Detailed Protocol

Why Choose AffiASSAY®?

Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.

AffiASSAY® Catalase Activity Colorimetric Assay Kit Protocol:

[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]

Materials Needed:

• AffiASSAY® Catalase Activity Colorimetric Assay Kit
• Biological samples containing catalase
• 96-well microplate
• Microplate reader capable of measuring absorbance at specific wavelengths
• Pipettes and tips
• Microcentrifuge tubes

Protocol:

Standard Curve Preparation:

• Reconstitute the Catalase Standard provided in the kit according to the kit's instructions.
• Create a series of standard dilutions using the reconstituted Catalase Standard to generate a standard curve. Typical concentrations may range from 0 to 1000 U/mL.

Assay Plate Setup:

• Label the microplate wells according to your experimental design, including wells for your samples, standards, and blanks.
• Prepare blank wells containing assay buffer only.

Sample and Standard Addition:

• Add 50-100 µL of your prepared samples and Catalase Standards to the appropriate wells on the microplate.
• Ensure that the final reaction volume in each well is consistent.

Reaction Initiation:

• Add 50 µL of Catalase Substrate (Hydrogen Peroxide) to each well to initiate the reaction.

Reaction Termination:

• Add 50 µL of Potassium Hydroxide (KOH) to each well after a specified incubation period to stop the reaction.

Measurement:

• Measure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.

Data Analysis:

• Generate a standard curve using the absorbance values obtained from the Catalase Standards.
• Calculate the catalase activity in your samples based on their respective absorbance readings and the standard curve.

Results Interpretation:

• Interpret and analyze your results according to your research objectives.
• Report catalase activity as units per milliliter (U/mL) or units per milligram of protein (U/mg protein) if protein normalization is performed.

Data Presentation:

• Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.

Cleanup:

• Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
• Always refer to the specific instructions provided with the AffiASSAY® Catalase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.
• Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.