AffiNGS® VAHTS Universal V8 RNA-seq Library Prep Kit for MGI
AffiNGS® VAHTS Universal V8 RNA-seq Library Prep Kit for MGI is specially designed for the preparation of transcriptome libraries for next generation sequencing (NGS) platforms of MGI®. The kit is universal and suitable for RNA library construction of RNA that have been obtained by Poly(A)-based mRNA enrichment or rRNA depletion. The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library construction for non-stranded or stranded RNA-Seq transcriptome analysis.
This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with
no need of purification, which greatly simplifies the process of library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained after size selection with magnetic beads. All the enzymes and buffer provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability.
Components:
| Components |
AFG-VZ-NRM605-01 (24 rxns) |
AFG-VZ-NRM605-02 (96 rxns) |
| 2 X Frag/Prime Buffer | 240 µl | 960 µl |
| 1st Strand Buffer 3 | 168 µl | 672 µl |
| 1st Strand Enzyme Mix 3 | 48 µl | 192 µl |
| 2nd Strand Buffer 2 (with dNTP) | 600 µl | 4 X 600 µl |
| 2nd Strand Buffer 2 (with dNTP) | 600 µl | 4 X 600 µl |
| 2nd strand Enzyme super Mix 2 | 360 µl | 2 X 720 µl |
| Rapid Ligation Buffer 4 | 600 µl | 4 X 600 µl |
| Rapid DNA Ligase 4 | 120 µl | 480 µl |
| PCR Primer Mix 4 | 120 µl | 480 µl |
| 2 X HF Amplification Mix | 600 µl | 4 X 600 µl |
Storage:
Store at -30 ~ -15℃ and transport at ≤0℃.