AffiNGS® Ribo-off rRNA Depletion Kit (Human/Mouse/Rat)
AffiNGS® Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) is designed for removing ribosomal RNA (rRNA) (including cytoplasmic 28S, 18S, 5.8S, 5S rRNA, and mitochondrial 16S, 12S rRNA) from total RNA of humans, mice, and rats while retaining mRNA and other non-coding RNA.
The kit is applicable to both intact and partially degraded RNA samples (e.g. FFPE RNA), and the resulting ribosomal-depleted RNA can de used for the analysis of mRNA ans non-coding RNA (such as IncRNA).
Components:
| Components | AFG-VZ-N406-01 (24 rxns) | AFG-VZ-N406-02 (96rxns) |
| rRNA Probe (H/M/R) | 24 µl | 96 µl |
| Probe Buffer | 72 µl | 288 µl |
| RNase H Buffer | 96 µl | 384 µl |
| Rnase H | 24 µl | 96 µl |
| Dnase I Buffer | 696 µl | 4 x 696 µl |
| Dnase I | 24 µl | 96 µl |
| Nuclease-free ddH2O | 1 ml | 4 x 1 ml |
Storage:
Store at -30 ~ -15°C and transport at ≤0°C.