AffiCELL® CCK-8 Cell Counting Kit is based on WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] that is rapidly absorbing, highly sensitive and widely used in detection of cell proliferation and cytotoxicity. The amount of formazan dye generated by dehydrogenases in living cells is directly proportional to the number of living cells. Measure the Optical Density at 450 nm, using a microplate reader. The measurements can be indirectly shows the number of living cells. AffiCELL® CCK-8 Cell Counting Kit contains CCK-8 Solution, which is a ready-to-use reagent. AffiCELL® CCK-8 Solution can be directly added to cell samples. After Incubating the mixed solution for a certain period of time, the assay can be performed without preparing various components in advance.
Storage Conditions:
Store at 2 ~ 8℃ and protect from light. Adjust the shipping method according to the destination
Application:
It is applicable for cell proliferation assay, cytotoxicity assay and drug screening, etc.
Self-prepared Materials:
Multi-channel pipettes (100 - 200 μl, 10 μl), microplate reader (with a 450 nm filter), 96 well plate, CO2 incubator.
Notes:
- CCK-8 Solution is pink solution, please protect from light.
- For the initial experiment, it is recommended to explore the optimal number of cell seeding and the optimal incubation time of CCK-8 Solution (General incubation time is 1 - 4 h).
- Be careful not to introduce bubbles to the wells.
- The assay of this kit depends on the reaction catalyzed by dehydrogenase, so oxidizing and reducing agents will interfere with the assay.Please remove interfering substances before use and then assay with CCK-8 Solution.
- If the drug contains metals (Pb2+, Fe2+, Cu2+, etc.), it will interfere with the color reaction of CCK-8 Solution, resulting in the decrease of detection sensitivity.
- When measuring cell number, in order to ensure the stability and repeatability of the test results, it recommended drawing a standard curve at the same time.