AffiAB® Goat Anti-Rab35 Polyclonal IgG Antibody
AffiAB® Goat Anti-Rab35 Polyclonal IgG Antibody is a top-quality polyclonal antibody, developed to provide optimum performance in western blotting and ELISA. Our antibody is highly specific for Rab35 and has a high affinity for binding to the antigen. Use this antibody for reliable, reproducible results.
Host: Goat
Species: Mouse
Description:
The AffiAB® Goat Anti-Rab35 Polyclonal IgG Antibody is an antibody specifically designed to recognize and bind to the Rab35 protein. Rab35 is a small GTPase that belongs to the Rab family of proteins, which play crucial roles in regulating intracellular membrane trafficking and vesicle transport.
This polyclonal antibody is produced by immunizing goats with purified Rab35 protein or a specific peptide derived from Rab35. The resulting antibodies are then purified from goat serum to ensure high specificity and quality.
The AffiAB® Goat Anti-Rab35 Polyclonal IgG Antibody is widely used in research studies to investigate the expression, localization, and function of Rab35 in various cellular processes. By specifically detecting and visualizing Rab35, researchers can gain insights into its involvement in membrane trafficking, endocytosis, cytokinesis, and cell signaling.
Researchers employ techniques such as immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation with this antibody to analyze Rab35 protein expression, subcellular distribution, and interactions with other proteins or cellular components.
It is important to note that the AffiAB® Goat Anti-Rab35 Polyclonal IgG Antibody specifically targets Rab35 and may not cross-react with other Rab proteins or related molecules. Researchers should validate the antibody's performance and specificity in their specific experimental conditions by conducting appropriate controls and assays.
In summary, the AffiAB® Goat Anti-Rab35 Polyclonal IgG Antibody is a valuable tool for studying the expression, localization, and function of Rab35 in cellular processes, particularly in the context of membrane trafficking and vesicle transport. By specifically detecting Rab35, researchers can advance our understanding of intracellular processes and their regulation.
Gene Identifier/Accession Number: ENSG00000111737
Form: N/A
Concentration: 2.5 mg/mL
Type: Primary
Clonality: Polyclonal
Isotype: IgG
Conjugation: Unconjugated
Application: Western Blot
Storage: For continuous use, store at 2-8 °C for one-two days. For extended storage, store in -20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.
Goat Anti-Rab35 Polyclonal IgG Antibody
In the realm of cell biology, understanding the intricate mechanisms of intracellular trafficking is essential for unraveling the mysteries of cellular function. Rab35, a member of the Rab GTPase family, is a critical regulator of vesicular trafficking and membrane dynamics. The Goat Anti-Rab35 Polyclonal IgG Antibody has emerged as a valuable research tool, enabling scientists to delve deeper into the complexities of Rab35-mediated intracellular transport and its implications in cellular processes and diseases.
Exploring the Role of Rab35
Rab35 is a small GTPase protein that plays a pivotal role in regulating membrane trafficking events, including endocytosis, exocytosis, and recycling of membrane vesicles. It acts as a molecular switch, cycling between an active GTP-bound state and an inactive GDP-bound state to control vesicle formation, movement, and fusion with target membranes. Rab35 is involved in various cellular processes, including cytokinesis, phagocytosis, and cell migration, making it a central player in cell biology.
Goat Anti-Rab35 Polyclonal IgG Antibody: An Overview
The Goat Anti-Rab35 Polyclonal IgG Antibody is specifically designed to target Rab35 proteins. This antibody is produced by immunizing goats with purified Rab35 protein, resulting in a polyclonal mixture of immunoglobulins G (IgG) capable of recognizing multiple epitopes on the Rab35 molecule. Its polyclonal nature enhances its versatility, making it an invaluable asset for a wide range of experimental applications.
Applications of Goat Anti-Rab35 Polyclonal IgG Antibody
Immunofluorescence (IF) and Immunohistochemistry (IHC):
Researchers utilize this antibody in IF and IHC experiments to visualize the subcellular localization of Rab35 within cells and tissues. These techniques enable the assessment of changes in Rab35 distribution under different biological contexts.
Western Blotting:
The antibody is used in Western blot assays to quantify Rab35 protein levels in cell lysates or tissue extracts. This application is instrumental for studying changes in Rab35 expression and post-translational modifications under various experimental conditions.
Immunoprecipitation (IP):
Scientists employ this antibody in IP assays to isolate Rab35-associated protein complexes, shedding light on its interactions with other molecules and its involvement in specific cellular processes.
Functional Studies:
The Goat Anti-Rab35 Polyclonal IgG Antibody can be applied in functional assays to investigate the impact of Rab35 modulation on vesicular trafficking, membrane dynamics, and cellular homeostasis, providing crucial insights into its roles in intracellular transport.
Disease Research:
Dysregulation of Rab35 has been linked to various diseases, including cancer, neurodegenerative disorders, and infectious diseases. Researchers use this antibody to explore the underlying mechanisms and potential therapeutic targets associated with Rab35-related pathologies.
Conclusion
The Goat Anti-Rab35 Polyclonal IgG Antibody is an invaluable asset in cell biology and cellular transport research. Its specificity, versatility, and reliability make it an indispensable tool for investigating the functions of Rab35 in cellular processes and understanding its implications in health and disease. As scientists continue to uncover the intricacies of intracellular trafficking pathways, this antibody remains a trusted partner in their quest for knowledge and advancements in cellular research and beyond.