AffiTaq® DNA Polymerase - With Buffer - With MgCl2 - Without dNTP

AffiTaq® DNA Polymerase - With Buffer - With MgCl₂ - Without dNTP

Product Overview:

Description: AffiTaq® DNA Polymerase is a high-quality enzyme blend formulated with a proprietary buffer solution and MgCl₂ for optimal PCR performance. This variant is tailored specifically for PCR applications where the addition of dNTPs is done separately, providing flexibility in experimental designs.

Product Compenents: 

Product Specifications:

Composition:

• DNA Polymerase: AffiTaq® sourced from highly purified recombinant enzymes, offering robust and reliable performance.
• Buffer: A unique proprietary buffer blend designed to optimize enzyme activity and stability during amplification reactions.
• MgCl₂ Concentration: Included at an optimized concentration for enhanced polymerase activity.

Storage Conditions:

• Temperature: Store at -20°C to ensure long-term stability and preservation of enzymatic activity.
• Stability: This product maintains stable activity for up to 24 months when stored under recommended conditions.

Performance Characteristics:

Key Features:

• Higher Robustness: AffiTaq® DNA Polymerase ensures high robustness and accuracy during DNA amplification.
• Enhanced Stability: The proprietary buffer composition and optimized MgCl₂ concentration ensure prolonged enzymatic stability.
• Versatile Usage: Suitable for various PCR applications, including but not limited to, routine PCR, endpoint PCR, and high-throughput PCR.

Applications:

• PCR Applications: Ideal for routine PCR, genotyping and other molecular biology applications requiring high-quality amplification.
• Sensitivity and Specificity: Demonstrates exceptional sensitivity and specificity across a wide range of templates and reaction conditions.

Quality Control:

QC Tests Performed:

• Stringent quality control tests are conducted, including purity assays, activity assays, and specificity assays, to guarantee consistent performance and reliability.

Quality Assurance:

• Manufactured under ISO-certified conditions with rigorous quality assurance protocols to ensure batch-to-batch consistency and reliability.

Usage Guidelines:

Recommended Protocol:

• Detailed protocols and guidelines for using AffiTaq® DNA Polymerase are provided, including recommended reaction conditions and cycling parameters for optimal results.
• Recommended Concentrations: General recommendations for primer and template concentrations are provided for various PCR applications.

Handling Precautions:

• Follow standard laboratory safety practices when handling enzymes. Avoid repeated freeze-thaw cycles to maintain enzyme activity.

Regulatory Compliance:

Certifications and Compliance:

• Complies with relevant regulatory standards and is manufactured in accordance with ISO 9001:2015 guidelines.
• Certified free of contaminants such as endonucleases, exonucleases, and RNases.

Safety Data:

• Safety data sheets (SDS) detailing hazard warnings, storage, handling, and disposal information are available upon request.

Packaging Information:

Packaging Details:

• Available in various packaging sizes to accommodate different laboratory requirements, with stringent sealing to prevent contamination.
• Packaging materials ensure stability and integrity during transportation and storage.

Labeling Information:

• Each product is labeled with batch numbers, expiration dates, storage conditions, and specific composition details for traceability and quality control purposes.

Suggested protocol for PCR Using AffiTaq® DNA Polymerase - With Buffer - With MgCl₂ - Without dNTP

Materials Provided:

1. AffiTaq® DNA Polymerase (5U/μL)
2. AffiTaq® Buffer (10X)
3. AffiTaq® MgCl₂ (25mM)

Materials Required but not provided:

1. Primer pairs specific to the target DNA sequence (25)
   
2. Template DNA
3. Nucleotides (dNTPs) (10 mM)
4. PCR tubes/strips and PCR machine
5. Sterile, nuclease-free water
6. PCR-grade pipettes and tips

Protocol:

Preparation:

1. Thaw the AffiTaq® DNA Polymerase set components on ice.
2. Prepare a master mix containing the following components for each PCR reaction:
   • AffiTaq® DNA Polymerase (5 U/μl)
   • AffiTaq® Buffer (10X)
   • MgCl₂ (if not included in the buffer, add as specified in the product instructions)
   • Sterile, nuclease-free water
   • Note: Exclude dNTPs from the master mix as they will be added separately.

PCR Reaction Setup:

1. Set up the PCR reaction tubes/strips on ice.

2. Add the following components to each PCR tube:

3. Mix the contents gently by pipetting and briefly spin down the tubes to collect all the liquid at the bottom.

PCR Cycling Conditions:

1. Start the PCR machine and set the following cycling parameters:

• Initial Denaturation: 95°C for 2-5 minutes (1 cycle)
• Denaturation: 95°C for 15-30 seconds
• Annealing: [Optimal temperature]°C for 15-30 seconds
• Extension: 72°C for [Extension time based on product guidelines] seconds
• Final Extension: 72°C for 5-10 minutes (1 cycle)
• Hold at 4-10°C

Post-PCR Steps:

1. Analyze the PCR products using agarose gel electrophoresis or other appropriate methods.

2. Store the remaining PCR products at -20°C for further analysis or downstream applications.

Notes:

• Use proper aseptic techniques and wear gloves to avoid contamination.
• Ensure adjustments to annealing temperature and extension time, if necessary, for specific primer-template combinations.
• Always refer to the specific instructions provided with the AffiTaq® DNA Polymerase set for optimal conditions based on your experiment.