AffiPCR® SYBR Color qPCR Master Mix (Low ROX Premixed)
AffiPCR® SYBR Color qPCR Master Mix (Low ROX Premixed) is a special 2X premix for qPCR assays based on SYBR Green I fluorescence method. The core component, Champagne Taq DNA Polymerase, is a novel antibody-modified hot-start DNA polymerase with high specificity and detection sensitivity. Equipped with the optimized buffer for qPCR and specificity-promoting factors, it is very suitable for highly specific and sensitive qPCR assays. This product is a 2X premix reagent that contains SYBR Green I at an optimal concentration for qPCR. It can obtain a good standard curve in a wide quantitative range, and accurately quantify target genes with good repeatability and high reliability. In addition, this product can track the pipetting process by using the color change effect produced by the mixing of different dyes, thereby significantly reducing pipetting errors.
Components:
| Components | 500 Reactions | 2500 Reactions |
| 2X SYBR Color qPCR Master Mix (Low ROX Premixed) | 4 × 1.25 mL | 5 X 4 × 1.25 mL |
| 10 × Dilution Buffer | 1.25 mL | 5 X .25 mL |
Application:
It is applicable for amplification reaction of animal, plant and microbial DNA.
Applicable Instruments:
This product is premixed with ROX Reference Dye 2 (low concentration) to correct the fluorescence signal error between wells. It is suitable for the following qPCR instruments:
Storage Conditions:
Store at -30°C ~ -15°C and protect from light. Transport at ≤0°C. After thawing, the Master mix can be stored stably for 6 months at 2°C ~ 8°C and protected from light.
Equivalence:
| AFG-VZ-Q411 | AffiGEN AffiPCR® SYBR Color qPCR Master Mix |
| AFG-VZ-Q421 | AffiGEN AffiPCR® SYBR Color qPCR Master Mix (Without ROX) |
| AFG-VZ-Q431 | AffiGEN AffiPCR® SYBR Color qPCR Master Mix (Low ROX Premixed) |
| AFG-VZ-Q441 | AffiGEN AffiPCR® SYBR Color qPCR Master Mix (High ROX Premixed) |
| K0392 | Thermo Fisher Luminaris Color HiGreen qPCR Master Mix |
| RR820 Q/A/B | TaKaRa TB Green®Premix Ex Taq™II(Tli RNaseH Plus) |
| 4913850001 | Roche FastStart Universal SYBR Green Master |
| 4472908 | ABI SYBR Select Master Mix |
| 208052 | Qiagen QuantiNova SYBR Green PCR Kit |