AffiPCR® Select RT SuperMix for qPCR V2
AffiPCR® HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The HiScript II Q Select RT SuperMix for qPCR is specially designed for two-step qRT-PCR. 5 × HiScript II Select qRT SuperMix contains Buffer, dNTP, HiScript II Reverse Transcriptase and RNase inhibitor. Oligo (dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. Oligo (dT)23VN has stronger anchoring ability to Poly A+ mRNA than Oligo(dT)18, making reverse transcription more efficient. 5X HiScript II Select qRT SuperMix does not freeze at -20°C, and the reaction proceeds quickly after adding template RNA and primers. The RT product is compatible with dye-based and probe-based qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for high-performance gene expression analysis.
Components:
| Component |
Quantity (20 μl/Reaction) |
| RNase-free ddH2O | 2 × 1 mL |
| 5X HiScript II Select qRT SuperMixa | 400 μL |
| Oligo (dT)23VN (10 μM) | 100 μL |
| Random hexamers (50 ng/μl) Components | 100 μL |
| 5X Select No RT Control Mixb | 40 μL |
Equivalence:
| AFG-VZ-R232 | AffiGEN AffiPCR® Select RT SuperMix for qPCR V2 |
| RR037 | TaKaRa PrimeScript™ RT reagent Kit (Perfect Real Time) |