AffiPCR® One Step qRT-PCR Probe Kit V2
AffiPCR® One Step qRT-PCR Probe Kit V2 is specially designed for qPCR detection using RNA (e.g., RNA virus) as templates. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube. No additional opening/pipetting operations are required, greatly increasing assay throughput and reducing the risk of contamination. Combining the superior performance of HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, equipping with an optimized buffer, the detection sensitivity of HiScript II One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or <10 copies of RNA templates. This product is provided as master mix. The 2X One Step Mix contains an optimized buffer and dNTP mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g., TaqMan).
Components:
| Component |
AFG-VZ-Q223-EN01 250 rxns (20 μl/rxn) |
| RNase-free ddH2O | 2 × 1.25 ml |
| 2X One Step Mixa | 2 × 1.25 m |
| One Step Enzyme Mixb | 250 μl |
| 50X ROX Reference Dye 1c | 100 μl |
| 50X ROX Reference Dye 2c | 100 μl |
a. It contains dNTP Mix and Mg2+.
b. It contains HiScript II Reverse Transcriptase, RNase inhibitor and Champagne Taq DNA Polymerase.
c. It is used to correct the error of fluorescence signals between wells. Use 50X ROX Reference Dye 1 for ABI 7900HT/7300 Real-Time PCR System and StepOnePlus; Use 50X ROX Reference Dye 2 for ABI 7500, 7500 Fast Real-Time PCR System, and Stratagene Mx3000P. Don't use ROX for Roche and Bio-Rad Real-Time PCR instruments.
Storage:
Store at -30°C ~ -15°C and transport at ≤0°C.
Applications:
This product is suitable for detection of various RNA nucleic acids of animals, plants and microorganisms (viruses, etc.).