AffiPCR® One Step qRT-PCR Probe Kit V1
AffiPCR® One Step qRT-PCR Probe Kit V1 is specially designed for qPCR detection using RNA (e.g., RNA virus) as templates. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube. No additional opening/pipetting operations are required, greatly increasing assay throughput and reducing the risk of contamination. Combining the superior performance of HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, equipping with an optimized buffer, the detection sensitivity of HiScript II One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or <10 copies of RNA templates. This product is provided as master mix. The 2X One Step Mix contains an optimized buffer and dNTP mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g., TaqMan).
Components:
| Component |
AFG-VZ-Q2221 250 rxns (20 μl/rxn) |
| RNase-free ddH2 O | 2 × 1.25 ml |
| 2X One Step Mixa | 2 × 1.25 ml |
| One Step Enzyme Mix b | 250 μl |
| 50X ROX Reference Dye 1c | 100 μl |
| 50X ROX Reference Dye 2c | 100 μl |
a. It contains dNTP Mix and Mg²+ .
b. It contains HiScript II Reverse Transcriptase, RNase inhibitor and Champagne Taq DNA Polymerase.
c. It is used to correct the error of fluorescence signals between wells. Use 50 × ROX Reference Dye 1 for ABI 7900HT/7300 Real-Time PCR System and StepOnePlus; Use 50 × ROX Reference Dye 2 for ABI 7500, 7500 Fast Real-Time PCR System, and Stratagene Mx3000P. Don't use ROX for Roche and Bio-Rad Real-Time PCR instruments.
Storage:
Store at -30°C ~ -15°C and transport at ≤0°C.
Equivalence:
| AFG-VZ-Q222 | AffiGEN AffiPCR® One Step qRT-PCR Probe Kit V1 |
| E3006 | NEB Luna® Universal Probe One-StepRT-qPCR Kit |
| RR064 | TaKaRa One Step PrimeScriptTM RT-PCRKit (Perfect Real Time) |
| A6120 | Promega GoTaq® Probe 1-Step RT-qPCR System |