SKU: AFG-YSG-03

AffiGEL® Matrix Phenol Red-Free, LDEV-Free

Vendor AffiGEN
Regular price $239.00 CAD
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AffiGEL® Matrix Phenol Red-Free, LDEV-Free

  • Equivalent: 356237 | Corning® Matrigel® Basement Membrane Matrix, Phenol Red-free, LDEV-free
  • Matrix type: Basic concentration (8-12 mg/ml)
  • Application: Color detection such as fluorescence detection experiments, etc

Product Information

AffiGEL® Matrix Phenol Red-Free, LDEV-Free, is recommended for assays that require color detection (e.g. fluorescence). Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

  • Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
  • Extensive PCR testing is performed to screen for a number of pathogens
  • Tested and found negative for bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
  • Biological activity is determined for each lot using a neurite outgrowth assay

Source

EHS mouse tumor

Application

  • Cell Growth and Differentiation
  • Metabolism/Toxicology Studies
  • Invasion Assays
  • in vitro and in vivo Angiogenesis Assays
  • in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.