AffiASSAY® Free Fatty Acid Colorimetric & Fluorometric Assay Kit
The AffiASSAY® Free Fatty Acid Colorimetric & Fluorometric Assay Kit offers a reliable method for the quantification of free fatty acids (FFAs) in various biological samples. This assay kit provides flexibility with options for both colorimetric and fluorometric detection, making it suitable for a wide range of research applications in lipid metabolism, metabolic diseases, and nutritional studies.
Key Features:
- Dual Detection: Our assay kit enables the measurement of FFAs using both colorimetric and fluorometric methods, offering versatility based on experimental needs.
- High Sensitivity: The kit can detect low levels of FFAs, ensuring accurate quantification in biological samples.
- Wide Application: The AffiASSAY® Free Fatty Acid Assay Kit is compatible with various sample types, including plasma, serum, tissues, cell lysates, and culture media.
- Quick and Easy: With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.
- Cost-Effective: Our kit provides an affordable solution for measuring FFAs, reducing experimental costs without compromising data quality.
Assay Principle:
The assay kit measures FFAs based on the enzymatic hydrolysis of fatty acids to produce a colorimetric or fluorometric signal. The signal intensity is directly proportional to the concentration of FFAs in the sample.
Applications:
- Lipid Metabolism Research: Study the regulation of lipid metabolism and the role of FFAs in various physiological processes.
- Metabolic Disease Studies: Investigate the association between FFA levels and metabolic disorders such as obesity, diabetes, and cardiovascular diseases.
- Nutritional Research: Assess the impact of dietary interventions and nutritional supplements on FFA metabolism and lipid profiles.
Kit Contents:
- Free Fatty Acid Assay Buffer
- Free Fatty Acid Enzyme Mix
- Free Fatty Acid Probe
- Microplate (96 wells)
- Free Fatty Acid Standard
- Detailed Protocol
Why Choose AffiASSAY®?
Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.
AffiASSAY® Free Fatty Acid Colorimetric & Fluorometric Assay Kit Protocol:
[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]
Materials Needed:
- AffiASSAY® Free Fatty Acid Colorimetric & Fluorometric Assay Kit
- Biological samples containing free fatty acids
- Microplate reader capable of measuring absorbance and fluorescence
- Pipettes and tips
- Microcentrifuge tubes
Protocol:
Standard Curve Preparation:
- Prepare a series of standard solutions containing known concentrations of the free fatty acid standard provided in the kit.
- Dilute the Free Fatty Acid Probe with Free Fatty Acid Assay Buffer to prepare the working solution.
- Add the working solution to each standard solution to initiate the reaction.
Assay Plate Setup:
- Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
- If using controls, prepare control samples without free fatty acids to serve as background controls.
Sample and Standard Addition:
- Add 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.
- Ensure that the final reaction volume in each well is consistent.
Assay Reaction:
- Add an equal volume of Free Fatty Acid Enzyme Mix to each well to initiate the reaction.
- Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.
Colorimetric and Fluorometric Measurement:
- After the incubation period, measure the absorbance and fluorescence of each well using a microplate reader.
- For colorimetric detection, measure the absorbance at the appropriate wavelength.
- For fluorometric detection, measure the fluorescence intensity using appropriate excitation and emission wavelengths.
Data Analysis:
- Generate standard curves using the absorbance or fluorescence values obtained from the standard solutions.
- Calculate the free fatty acid concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curves.
Results Interpretation:
- Interpret and analyze your results according to your research objectives.
- Observe any variations or trends in free fatty acid levels among your samples.
Data Presentation:
- Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.
Cleanup:
- Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
- Always refer to the specific instructions provided with the AffiASSAY® Free Fatty Acid Colorimetric & Fluorometric Assay Kit for any kit-specific details or variations in the protocol.
- Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.