AffiPCR® One Step qRT-PCR Probe Kit V3
AffiPCR® One Step qRT-PCR Probe Kit V3 is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit can prevent the contamination of PCR product. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability.
HiScript III One Step qRT-PCR Probe Kit is provided in Master Mix. The 5X One Step Mix contains an optimized buffer and dNTP Mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).
| Components |
AFG-VZ-Q225-EN01 100 rxns (30 μl/rxn) |
AFG-VZ-Q225-EN02 1,000 rxns (30 μl/rxn) |
AFG-VZ-Q225-EN03 5,000 rxns (30 μl/rxn) |
| RNase-free ddH2O | 2 X 1 ml | 20 ml | 100 ml |
| 5X One Step Mixa | 600 µl | 6 x 1 ml | 30 ml |
| One Step Enzyme Mixb | 150 µl | 2 x 750 µl | 7,5 ml |
| 50X ROX Reference Dye 1c | 60 µl | 600 µl | 3 x 1 ml |
| 50X ROX Reference Dye 2c | 60 µl | 600 µl | 3 x 1 ml |
Storage:
Store at -30°C ~ -15°C and transport at ≤0°C.