AffiAB® Chicken Anti-Chicken IgY, DyLight® 405 Polyclonal IgY Antibody
Our Chicken Anti-Chicken IgY, DyLight® 405 Polyclonal IgY Antibody is an ideal choice for research applications. Using our antibody, customers can conduct high-affinity immunodetection while significantly reducing background noise. Our experienced team of experts guarantees the most accurate results, making it ideal for any lab.
Host: Chicken
Species: Chicken
Description:
AffiAB® Chicken Anti-Chicken IgY, DyLight® 405 Polyclonal IgY Antibody is a polyclonal antibody produced in chickens that specifically recognizes and binds to the IgY antibodies found in chickens. This antibody has been conjugated to the fluorophore DyLight® 405, which emits a blue-violet fluorescence when excited by light at 405 nm.
The chicken IgY antibody is commonly used in research to detect and quantify the presence of chicken antibodies in samples, such as in ELISA assays, western blotting, and immunohistochemistry. The use of chicken antibodies has several advantages over traditional antibodies derived from mammals, including low cross-reactivity with mammalian antibodies and a lower risk of inducing an immune response in mammalian hosts.
The DyLight® 405 conjugation of the chicken anti-chicken IgY antibody provides enhanced sensitivity and specificity in immunofluorescence applications. The fluorophore has high photostability and minimal background noise, which makes it ideal for multicolor labeling experiments and imaging studies.
Overall, the Chicken Anti-Chicken IgY, DyLight® 405 Polyclonal IgY Antibody is a valuable tool for researchers working in the field of immunology and protein research.
Gene Identifier/Accession Number: N/A
Form: N/A
Concentration: 2.5 mg/mL
Type: Secondary
Clonality: Polyclonal
Isotype: IgY
Conjugation: DyLight® 405
Application: Western Blot, Immunofluorescence
Storage: For continuous use, store at 2-8 °C for one-two days. For extended storage, store in -20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.
What is Chicken Anti-Chicken IgY?
Chicken Anti-Chicken IgY is a type of immunoglobulin Y (IgY) antibody produced in chickens. IgY antibodies are the avian equivalent of the mammalian IgG. They are harvested from the egg yolk of chickens immunized with a specific antigen, in this case, chicken proteins. This method of antibody production is non-invasive and ethical, making it a preferred choice in many research settings.
The Significance of DyLight® 405 Conjugation
DyLight® 405 is a fluorophore used to label antibodies, allowing them to be visible under specific light conditions. When Chicken Anti-Chicken IgY is conjugated with DyLight® 405, it becomes an invaluable tool for fluorescence microscopy, flow cytometry, and other applications requiring high-intensity, stable fluorescence. This conjugation enhances the antibody's utility, especially in complex imaging and diagnostic procedures.
Applications in Research and Diagnostics
1. Fluorescence Microscopy:
The Chicken Anti-Chicken IgY, DyLight® 405 antibody, is extensively used in fluorescence microscopy. Its ability to bind to chicken proteins and emit a bright, stable fluorescence under UV light makes it a powerful tool for studying cellular and molecular processes.
2. Flow Cytometry:
In flow cytometry, this antibody assists in the accurate detection and analysis of chicken-derived cells and proteins. Its high fluorescence intensity ensures reliable and precise data collection.
3. Immunohistochemistry:
Used in immunohistochemistry, the antibody provides clear and specific staining of chicken proteins in tissue samples, aiding in the study of pathological conditions in avian species.
4. Western Blotting:
The Chicken Anti-Chicken IgY, DyLight® 405 antibody, is also useful in Western blotting for detecting chicken proteins with high sensitivity.
Advantages over Traditional Antibodies
The Chicken Anti-Chicken IgY, DyLight® 405 Polyclonal IgY Antibody offers several advantages over traditional mammalian antibodies:
- Ethical Production: Harvesting from egg yolks is non-invasive and more ethical compared to mammalian antibody production.
- Reduced Background Noise: IgY does not bind to Fc receptors in mammalian cells, reducing non-specific binding and background noise in experiments.
- Stability: The DyLight® 405 conjugation increases the stability and visibility of the antibody in various applications.