The AffiLINK® Biotin Dye Antibody Labeling Kit enables efficient antibody labeling within 30 minutes, with yields of up to 98%. This reliable and easy-to-use kit allows researchers to conduct experiments quickly and accurately.
The AffiLINK® Biotin Dye Antibody Labeling Kit is a cutting-edge solution designed by AffiGen to empower researchers with a simplified and efficient method for antibody labeling. This kit allows for the conjugation of biotin dyes to antibodies, enabling precise and sensitive detection in various immunoassays and imaging techniques.
Technical Description:
The AffiLINK® Biotin Dye Antibody Labeling Kit offers several key features that make it an ideal choice for researchers seeking reliable and reproducible antibody labeling:
1. High-Quality Biotin Dyes: The kit includes a selection of high-quality biotin dyes specifically optimized for antibody conjugation. These dyes exhibit excellent brightness, photostability, and minimal background noise, ensuring superior performance in downstream applications.
2. Efficient Conjugation Chemistry: The labeling process utilizes a robust and efficient conjugation chemistry that enables covalent attachment of biotin dyes to antibody molecules. This chemistry ensures stable and long-lasting conjugation, minimizing any potential loss of antibody functionality.
3. Versatile Compatibility: The AffiLINK® Biotin Dye Antibody Labeling Kit is compatible with a wide range of antibodies, including monoclonal and polyclonal antibodies derived from various species. This versatility allows researchers to label antibodies of interest for diverse applications, such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry.
General Protocol:
Follow this general protocol to achieve optimal results with the AffiLINK® Biotin Dye Antibody Labeling Kit:
1. Antibody Purification: Begin by purifying the antibody of interest using a suitable purification method to remove any contaminants or stabilizers that may interfere with the labeling process.
2. Dye Preparation: Prepare the biotin dye solution according to the provided instructions. Ensure proper handling and storage of the dye to maintain its integrity and performance.
3. Labeling Reaction: Combine the purified antibody with the biotin dye solution in the provided labeling buffer. Incubate the mixture under optimized conditions (temperature, time, and pH) to facilitate the conjugation reaction. Gentle agitation or mixing may be necessary to ensure thorough mixing of the components.
4. Removal of Unbound Dye: Following the conjugation reaction, remove any unbound dye molecules using the provided purification columns or other suitable methods. This step helps to enhance the specificity and signal-to-noise ratio of the labeled antibody.
5. Quality Control and Storage: Assess the labeling efficiency and quantify the concentration of the labeled antibody using appropriate analytical techniques. Store the labeled antibody as directed to maintain its stability and functionality.
Harness the Power of AffiLINK® for Precise Antibody Labeling
With the AffiLINK® Biotin Dye Antibody Labeling Kit, researchers gain access to a streamlined and reliable method for antibody labeling, ensuring precise detection in a variety of immunoassays and imaging techniques. Trust in the expertise of AffiGen to simplify your labeling process and enhance the sensitivity and accuracy of your research.
Unlock the potential of AffiLINK® and elevate your antibody labeling experiments. Contact our team of experts for technical support, personalized recommendations, and further guidance in utilizing the AffiLINK® Biotin Dye Antibody Labeling Kit to its fullest potential. Trust in AffiGen for innovative solutions that empower your scientific pursuits.