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SKU: AFG-SNG-TR-007

AffiGEN® Transfect(TM) DNA In Vitro 2000 Plus

Vendor AffiGEN
Regular price $244.00 AUD
Sale price $244.00 AUD Regular price
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AffiGEN® TransfectTM DNA In Vitro 2000 Plus

Product Description

AffiGEN® TransfectTM DNA In Vitro 2000 Plus represents an upgraded version of the GenJet™ DNA In Vitro Transfection Reagent. AffiGEN® TransfectTM DNA In Vitro 2000 Plus has been enhanced through the incorporation of a booster peptide, resulting in a remarkable up to 20 times higher transfection efficiency across a wide range of mammalian cells when compared to its previous iteration. AffiGEN® TransfectTM DNA In Vitro 2000 Plus has demonstrated its capability to efficiently deliver genes to various well-established cell lines, as well as primary cells, including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines such as Sf9 and Sf21, and various other eukaryotic cell lines, all with reduced toxicity. Each unit of AffiGEN® TransfectTM DNA In Vitro 2000 Plus reagent, with a volume of 1.0 ml, is sufficient for more than 600 transfections in 24-well plates or more than 300 transfections in 6-well plates.

Features

 - Achieves cell-dependent 3~20 times higher efficiency.
 - Remains the top choice for challenging-to-transfect cells.
 - Equally effective for very long DNA molecules (up to 46 kb).
 - Demonstrates increased efficiency even in the presence of serum and antibiotics for most cell types.
 - Yields exceptionally high levels of recombinant protein production.

Storage Condition

Store AffiGEN® TransfectTM DNA In Vitro 2000 Plus at 4°C. When stored properly, the product maintains stability for 12 months or longer.


DNA In Vitro Transfection Reagents Specifications

Product Nucleic Acid Type Delivery Method Toxicity Efficiency Serum Tolerance
AffiGEN® Transfect™ 1000 - Mammalian Cell DNA Calcium phosphate Precipitation High High High
AffiGEN® Transfect™ DNA In Vitro 2000 V1 DNA, siRNA Polymer Polymer complex Low High Low
AffiGEN® Transfect™ DNA In Vitro 2000 V2 DNA, siRNA Polymer Polymer complex Moderate Higher Medium
AffiGEN® Transfect™ DNA In Vitro 2000 Plus DNA Polymer Polymer complex Low Higher Medium
AffiGEN® Transfect™ DNA In Vitro 3000 DNA Liposomal Liposome-mediated Moderate High High
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit DNA, siRNA Liposomal Liposome-mediated Very low High High
AffiGEN® Transfect™ DNA In Vitro 5000 DNA, siRNA Polymer Polymer complex Moderate High High

 

DNA In Vitro Transfection Reagents Application Guide

Product Applications Cell Types Cell lines Suitable for Additional Notes
AffiGEN® Transfect™ 1000 - Mammalian Cell Protein production, stable cell line generation, viral vector production Adherent, some suspension HEK293, HepG2, CHO Large-scale transfection, cost-effective
Lower calcium concentration than traditional methods
AffiGEN® Transfect™ DNA In Vitro 2000 V1 Gene expression studies, protein production, virus production Most, including sensitive Various Sensitive cell lines, maximizing cell viability
Multiple formulations available
AffiGEN® Transfect™ DNA In Vitro 2000 V2 Gene expression studies, protein production, virus production Most, including sensitive Various Balancing efficiency and viability
Released from DNA complex
AffiGEN® Transfect™ DNA In Vitro 2000 Plus Protein production, viral vector production Most, including sensitive Various Long DNA, high efficiency
Proprietary polymer
AffiGEN® Transfect™ DNA In Vitro 3000 Viral vector production, antibody production, protein production Adherent, suspension Various Lentivirus production, high efficiency
Enhanced conjugate
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit Protein production, gene editing, virus production Most, including sensitive Various Low toxicity, high efficiency
Fluorinated cationic lipids
AffiGEN® Transfect™ DNA In Vitro 5000 Gene expression studies, protein production, virus production Most, including sensitive Various Common cell lines, biodegradable
Biodegradable polycationic polymer