SKU: AFG-CHK-0567

AffiCHECK® Staphylococcus aureus (mecA-) DNA - PCR Qualitative Positive Control

Vendor AffiGEN
Regular price $893.00 AUD
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AffiCHECK® Staphylococcus aureus (mecA-) DNA - PCR Qualitative Positive Control

Product Datasheet - AFG-CHK-0567

PRODUCT DESCRIPTION:

AffiCHECK® Staphylococcus aureus (mecA-) DNA - PCR Qualitative Control is a high-quality, reliable standard containing known quantities of Staphylococcus aureus (mecA-) DNA. It's designed using modified and non-infectious bacteria to ensure stability, thereby contributing to accurate and reliable results in PCR applications.

INTENDED USE:

AffiCHECK® Staphylococcus aureus (mecA-) DNA - PCR Qualitative Control serves as a comprehensive process control for the evaluation of the performance of nucleic acid tests for determination of the presence of Staphylococcus aureus (mecA-) nucleic acid. It's essential in validating the performance of PCR assays in clinical, research, and diagnostic settings.

PRODUCT FEATURES:

  • Specificity: Tailored for Staphylococcus aureus (mecA-) DNA detection.
  • Formulation: Lyophilized for enhanced stability.
  • Convenience: Ready-to-use upon reconstitution.
  • Compatibility: Suitable for a wide range of PCR assays.

CHARACTERISTICS:

  • Cultivation & Extraction: Cultivated in Mannitol Salt Agar and extracted using a reliable genomic DNA method.
  • Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
  • Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.

PERFORMANCE CHARACTERISTICS:

  • Sensitivity: Optimized for high sensitivity in PCR detection.
  • Specificity: Exclusively detects Staphylococcus aureus (mecA-) DNA.
  • Reproducibility: Consistent results across different test runs and batches.

PACK CONTENT :

Components Pack Size Note 
DNA Control Lyophilized Powder (1x104 to 2x104 Copies/µL) 1 Vial Quantified by Real-Time PCR.
Buffer 500 µL Water, DNase, RNase Free.

PRECAUTIONS:

  • For research use or diagnostic control purposes only.
  • Follow standard laboratory practices and use PPE.
  • Use Sterile Filter Tips to prevent contamination.
  • Handle infectious specimens with precautions.
  • Disinfect work surfaces.
  • Maintain separate working areas for testing.
  • Dispose of unused reagents and waste as per regulations.
  • May contain genetic material, handle with care.
  • No method ensures total absence of infectious agents.
  • Follow local waste disposal rules.
  • Dilute below 103 copies/µL immediately before use.
  • Avoid freezing for low-copy dilutions to prevent DNA degradation.

INSTRUCTIONS FOR USE:

  • Open the foil pouch of AffiCHECK® Staphylococcus aureus (mecA-) DNA.
  • Centrifuge AffiCHECK® Staphylococcus aureus (mecA-) DNA Lyophilized Powder at 1000 g for 1 minute.
  • Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
  • The concentration will be 1x104 to 2x104 Copies/µL once reconstituted.
  • Vortex for 1 min for complete dissolution and homogenization.

RECOMMENDED STORAGE:

  • Before Reconstitution: Store at 2-8ºC.
  • After Reconstitution: Store at -20ºC.
  • Shipping: No special instructions
  • Shelf Life: 12 months from production date.

ORDERING INFORMATION :

Catalog Number Description Size
AFG-CHK-0567 AffiCHECK® Staphylococcus aureus (mecA-) DNA PCR Qualitative Positive Control 1 vial + 500 µL Buffer